Histone demethylase KDM6B has an anti-tumorigenic function in neuroblastoma by promoting differentiation

a–c Kdm6b expression is downregulated in mouse neuroblastoma sphere-forming cells as measured by microarray (a), qRT-PCR (b, error bars, s.d., n？=？3), and immunoblotting (c), in comparison with primary tumor cells. α-tubulin levels are shown as loading control (c). Data were analyzed using two-tailed Student’s t-test. d–e Low KDM6B expression is associated with reduced event-free survival in neuroblastoma patients (d), high-risk neuroblastoma (e, left panel), and advanced stages of the tumors (e, right panel). Patient data analyses (d–e) were conducted online (R2 Genomics Analysis and Visualization Platform), and the resulting figures and log-rank test (d) and Student’s t-test (e) p values were downloaded. **p？<？0.01; ***p？<？0.00