Dihydroartemisinin inhibits prostate cancer via JARID2/miR-7/miR-34a-dependent downregulation of Axl

a qRT-PCR for Axl in PCa cells treated with 5？μM of DHA for 24？h. Values were normalized to Gapdh levels and to DMSO control. The experiment was performed in triplicate. Data are representative of 3 independent experiments; *p？<？0.05 two-tailed Student’s t-test. b Immunoblot analysis of protein extracts obtained from DU145 and PC-3 treated with 5？μM DHA for 24？h using anti-phospho-Axl, anti-phospho-Akt and anti-phospho-Stat3 and anti-Axl, anti-Akt, anti-Stat3 and anti- GAPDH. c Migration and d invasion analysis of mCRPC cell lines 24？h post treatment with 5？μM of DHA. Cells were fixed and stained, and 3–5 random microscopic fields were counted. Data are representative of three independent experiments and all values shown are mean？±？SD from a representative experiment; **p？<？0.01, two-tailed Student’s t-test. e Apoptosis assay for mCRPCa cell lines treated with 5？μM of DHA for 8？h. DMSO 0.05% was used as a control. Data are representative of three independent experiments and all values shown are mean？±？SD from a representative experiment; *p？<？0.05, two-tailed Student’s t-tes