Relative contributions of adipose-resident CD146+ pericytes and CD34+ adventitial progenitor cells in bone tissue engineering

Microcomputed tomographic (microCT) imaging and analysis of calvarial defect healing with CD146+ pericytes or CD34+ adventitial cells, alone or in combination. a 3D coronal section reconstructions of calvarial defect site among “no scaffold” control, “scaffold alone“ control, or three different cell treatment groups. “Pericyte” group consisted of 2.5？×？105 CD146+CD34？CD45？CD31？ pericytes. “Adventitial” group consisted of 2.5？×？105 CD34+CD146？CD45？CD31？ adventitial cells. “Pericyte？+？Adventitial” group consisted of a 50%/50% split between each cell type (1.25？×？105 adventitial cells and 1.25？×？105 pericytes). b–d MicroCT quantification of bone repair in calvarial defects within each treatment group, 8 weeks postoperative. b Bone volume, c bone volume/ttissue volume, and d Bone fractional area. *P？<？0.05, **P？<？0.01 in comparison with “no scaffold” group. N？=？46 bone defects per treatment grou