Encapsulation of macrophages enhances their retention and angiogenic potential

Optimisation of capsule generation. a The diameter of alginate capsules generated under a range of alginate concentrations (0.8% and 1.0%), and encapsulator flow rates (2–12？ml/min) was measured microscopically (n？=？10/group). b Variation of the encapsulator voltage settings influenced the diameter of capsules generated (n？=？5/group, error bars？=？s.d.). c Consistently round 300？μm diameter capsules were generated using 1.0% alginate with a flow rate of 12？ml/min and were used to encapsulate Tie2-iBMMs at a cell density of 1？×？107？cells/ml. Scale bar？=？100？μ