Opposing activities of oncogenic MIR17HG and tumor suppressive MIR100HG clusters and their gene targets regulate replicative senescence in human adult stem cells

Schematic representation of sample collection for analysis and immunological stability of hADSCs upon replicative SEN. a hADSCs were isolated from healthy donor subcutaneous tissue and passaged ex-vivo as described in materials and methods. Colorimetric detection of SA-β-Gal (10x) in SR and SEN hADSCs is shown. Samples were collected and processed for transcriptomic and proteomic studies as described in material and methods. b The table summarizes immunostability of hADSCs in SR and senescent states, which were assessed by expression of MSC-positive and MSC-negative CD markers, details shown in Supplementary Table S