Recent advances in the production of recombinant glycoconjugate vaccines

Traditional chemical conjugation method for the production of glycoconjugate vaccines. Multiple steps are required whereby the O-antigen must be purified from the pathogen of interest, detoxified and subject to chemical activation. In parallel, the protein must also be purified and chemically activated before protein and glycan can be conjugated. Following conjugation, further rounds of purification are necessary before vaccine can be administere