AgBR1 antibodies delay lethal Aedes aegypti-borne West Nile virus infection in mice

AgBR1 antiserum protects mice from mosquito-borne WNV virus infection. a The schematic of the experiment. Mice were administrated AgBR1 antiserum one day before WNV-infected mosquito feeding. Immunized mice were monitored for survival for 10 days after infected mosquito feeding. b The virus levels in blood of mice fed by an infected mosquito. Blood was collected every other day for 7 days from mice fed on by WNV-infected mosquitoes and analyzed by qRT-PCR. WNV RNA levels were normalized to mouse β actin RNA levels. Mice immunized with na？ve serum served as controls. Error bars represent mean？±？SEM. Each data point represents one mouse. Normalized viral RNA levels were analyzed using one-tailed Wilcoxon–Mann–Whitney test (n？=？13/each group biologically independent samples pooled from three separate experiments). c The weight of mice fed by an infected mosquito. Mice were monitored daily after WNV infection. Error bars represent mean？±？SEM. Weight at each time point were compared using one-tailed Wilcoxon–Mann–Whitney test (n？=？13/each group biologically independent samples pooled from three separate experiments). d Survival was assessed by a Gehan–Wilcoxon test (n？=？13/each group biologically independent samples pooled from three separate experiments