Determination of triacylglycerol oxidation mechanisms in canola oil using liquid chromatography–tandem mass spectrometry

Triacylglycerol (TG) oxidation mechanisms and the structures of triacylglycerol hydroperoxide isomers (a). Dioleoyl-linoleoyl glycerol (I) is oxidized to dioleoyl-(hydroperoxy octadecadienoyl) glycerol (OO-HpODE-TG, II–VII) by radical and/or singlet-oxygen oxidation. Hydroperoxide positions and geometrical structures depend on the TG peroxidation mechanisms. Schemes for the reference preparation of OO-HpODE-TG isomers (b). The references were synthesized from dioleoyl glycerol and HpODE isomer