Ubiquitous overexpression of the DNA repair factor dPrp19 reduces DNA damage and extends Drosophila life span

dPrp19 is the Drosophila melanogaster ortholog of human PRP19/SNEV/hPso4. a Prp19 is well-conserved from D. melanogaster to humans, as shown in an alignment of the protein sequences of D. melanogaster dPrp19 and human PRP19/SNEV/hPso4. The two orthologs have an amino acid identity of 66% and a sequence similarity of 81%. The human site of ATM phosphorylation, Ser149, is substituted for Gln in Drosophila (indicated by the black arrow). b dPrp19 expression is higher in female D. melanogaster and decreases with age. Comparison of dPrp19 mRNA levels in male (gray) and female (black) wild-type flies at 6, 14, 21, and 28 days of adulthood shows that dPrp19 levels decrease in females between days 21 and 28. Values represent means across three biological replicates (±1 standard deviation), normalized to Gapdh2 and Tubulin and average