SMARCD1 regulates senescence-associated lipid accumulation in hepatocytes

Functional analysis of SMARCD1 role in cellular senescence. Relative gene expression of SMARCD1 in presenescnet and senescent TIG-1 cells (a), in scramble shRNA (scr-shRNA)-transduced and SMARCD1-silenced presenescent TIG-1 cells (b), and in mock-transduced and SMARCD1-overexpressing senescent TIG-1 cells (F). (c) Expression of SMARCD1 in scr-shRNA-transduced and SMARCD1-silenced presenescent TIG-1 cells. (d) Proliferation rate of scr-shRNA-transduced (○) and SMARCD1-silenced presenescent TIG-1 (●, shSMARCD1-1; ■, shSMARCD1-2). Cellular senescence phenotypes, quantitatively analyzed by IN Cell analyzer 1000. Activity and expression of senescence markers were measured in scr-shRNA-transduced and SMARCD1-silenced presenescent TIG-1 cells (e), and in mock-transduced and SMARCD1-overexpressing senescent TIG-1 cells (h). (g) Proliferation rate of mock- (○) and SMARCD1-transduced TIG-1 cells (●). Data were analyzed by two-sided Student’s t-test and expressed as mean？±？SD. P？<？0.05 was considered significant (* P？<？0.05; ** P？<？0.01; *** P？<？0.001