Neuroprotective role of retinal SIRT3 against acute photo-stress

Retinal SIRT3 was mainly expressed in mitochondria, and the level was decreased with age. a, b In situ hybridization of Sirt3 in the retina of 10-week-old C57BL6/J mice. Scale bars: a 20？μm b 200？μm. GCL ganglion cell layer, IPL inner plexiform layer, INL inner nuclear layer, OPL outer plexiform layer, ONL outer nuclear layer, OS outer segment, RPE retinal pigment epithelium, AS antisense, S sense. c Representative Immunoblot of SIRT3 for subcellular fraction of the retina of 10-week-old C57BL6/J mice. T total retina, C cytoplasmic, M mitochondrial, N nuclear, CM cytoplasmic and mitochondrial fractions. d–g Immunoblot of young (10 weeks old) and old (60 weeks old) C57BL6/J mice retina against nicotinamide phosphoribosyltransferase (NAMPT) e, SIRT1 f, and SIRT3 g (n？=？5 for each group). *p？<？0.05 by two-tailed unpaired t-test. Values are mean？±？S