Platinum nanoparticles were electrodeposited on graphene modified glassy carbon electrode to form a modified electrode, and the electrode was characterized with scanning electron microscopy (SEM). At the modified electrode, rutin, a natural flavonoid, shows a couple of well-defined redox peaks, which is corresponded to the reduction and reoxidation of rutin. The electrochemical behaviors of rutin at the electrode were investigated, and the results indicated that the electrode reaction is controlled by adsorption process. Under the optimal conditions, the peak currents of differential pulse voltammetry (DPV) increased linearly with the rutin concentration in the range from to ？M with a limit of detection of ？M. The as-prepared electrode was successfully used for the selective determination of rutin in tablet, displaying a potential application of graphene composite modified electrode. 1. Introduction Rutin (quercetin-3-rutinoside, which chemical structure is shown in Figure 1) is a natural flavonoid with a wide of biochemical and pharmacological activities, including antigenotoxic, anti-inflammatory, antioxidant effects, antibacterial, antiviral, cytoprotective, and antiprotozoal properties, hypolipidaemic and anticonvulsive effects, and anticarcinogenic [1–6]. The interest in using rutin in cosmetic and pharmaceutical formulations is to enhance their antioxidant and vasoprotective properties, promoting relief of the symptoms of lymphatic and venous insufficiency. Up to now, methods for investigation of rutin have included spectrophotometry [7], capillary electrophoresis [8], chemiluminescence [9], HPLC [10], and electrochemical methods [11, 12]. Among these methods, electrochemical method is simple and sensitive. However, the sensitivity and selectivity of electrochemical method usually depends on the electrode material. Figure 1: Rutin chemical structure. Metal nanoparticles have attracted considerable attention due to their conspicuous physical and chemical properties [13, 14]. Previous investigates suggested that platinum nanoparticles have a good electrocatalytic activity among metal nanoparticles. So, platinum nanoparticles have widely been applied in many fields, such as electrocatalytic oxidation of formic acid and methanol [15, 16]. However, in order to utilize its function, platinum nanoparticles must be immobilized on supported materials, such as carbon materials and other conducting or semiconducting materials. Graphene is a new form of carbon material with carbon atoms parked in a two-dimensional honey lattice, which has attracted a
References
[1]
A. A. Ramos, C. F. Lima, M. L. Pereira, M. Fernandes-Ferreira, and C. Pereira-Wilson, “Antigenotoxic effects of quercetin, rutin and ursolic acid on HepG2 cells: evaluation by the comet assay,” Toxicology Letters, vol. 177, no. 1, pp. 66–73, 2008.
[2]
R. Guo, P. Wei, and W. Liu, “Combined antioxidant effects of rutin and Vitamin C in Triton X-100 micelles,” Journal of Pharmaceutical and Biomedical Analysis, vol. 43, no. 4, pp. 1580–1586, 2007.
[3]
K. H. Janbaz, S. A. Saeed, and A. H. Gilani, “Protective effect of rutin on paracetamol- and CCl-induced hepatotoxicity in rodents,” Fitoterapia, vol. 73, no. 7-8, pp. 557–563, 2002.
[4]
S. Y. Park, S. H. Bok, S. M. Jeon et al., “Effect of rutin and tannic acid supplements on cholesterol metabolism in rats,” Nutrition Research, vol. 22, no. 3, pp. 283–295, 2002.
[5]
M. Nassiri-Asl, S. Shariati-Rad, and F. Zamansoltani, “Anticonvulsive effects of intracerebroventricular administration of rutin in rats,” Progress in Neuro-Psychopharmacology and Biological Psychiatry, vol. 32, no. 4, pp. 989–993, 2008.
[6]
R. P. Webster, M. D. Gawde, and R. K. Bhattacharya, “Protective effect of rutin, a flavonol glycoside, on the carcinogen-induced DNA damage and repair enzymes in rats,” Cancer Letters, vol. 109, no. 1-2, pp. 185–191, 1996.
[7]
A. V. Pastukhov, L. A. Levchenko, and A. P. Sadkov, “Spectroscopic study on binding of rutin to human serum albumin,” Journal of Molecular Structure, vol. 842, no. 1–3, pp. 60–66, 2007.
[8]
Q. H. Lu, C. D. Ba, and D. Y. Chen, “Investigating noncovalent interactions of rutin—serum albumin by capillary electrophoresis—frontal analysis,” Journal of Pharmaceutical and Biomedical Analysis, vol. 47, no. 4-5, pp. 888–891, 2008.
[9]
D. A. Xu, Z. L. Gao, N. A. Li, and K. E. A. Li, “Tris(2,2′-bipyridyl)ruthenium(II) electrochemiluminescence (ECL) enhanced by rutin on platinum electrode,” Chinese Chemical Letters, vol. 18, no. 5, pp. 561–564, 2007.
[10]
K. Ishii, T. Furuta, and Y. Kasuya, “Determination of rutin in human plasma by high-performance liquid chromatography utilizing solid-phase extraction and ultraviolet detection,” Journal of Chromatography B, vol. 759, no. 1, pp. 161–168, 2001.
[11]
Y. A. Zhang and J. Zheng, “Sensitive voltammetric determination of rutin at an ionic liquid modified carbon paste electrode,” Talanta, vol. 77, no. 1, pp. 325–330, 2008.
[12]
A. A. Ensafi and R. Hajian, “Determination of rutin in pharmaceutical compounds and tea using cathodic adsorptive stripping voltammetry,” Electroanalysis, vol. 18, no. 6, pp. 579–585, 2006.
[13]
X. Dai, O. Nekraseova, M. E. Hyde, and R. G. Compton, “Anodic stripping voltammetry of arsenic(III) using gold nanoparticle-modified electrodes,” Analytical Chemistry, vol. 76, no. 19, pp. 5924–5929, 2004.
[14]
S. Hrapovic, Y. Liu, and J. H. T. Luong, “Reusable platinum nanoparticle modified boron doped diamond microelectrodes for oxidative determination of arsenite,” Analytical Chemistry, vol. 79, no. 2, pp. 500–507, 2007.
[15]
Z. H. Wang and K. Y. Qiu, “Electrocatalytic oxidation of formic acid on platinum nanoparticle electrode deposited on the nichrome substrate,” Electrochemistry Communications, vol. 8, no. 7, pp. 1075–1081, 2006.
[16]
S. Palmero, A. Colina, E. Mu？oz, A. Heras, V. Ruiz, and J. López-Palacios, “Layer-by-layer electrosynthesis of Pt-Polyaniline nanocomposites for the catalytic oxidation of methanol,” Electrochemistry Communications, vol. 11, no. 1, pp. 122–125, 2009.
[17]
Y. Xu, H. Bai, G. Lu, C. Li, and G. Shi, “Flexible graphene films via the filtration of water-soluble noncovalent functionalized graphene sheets,” Journal of the American Chemical Society, vol. 130, no. 18, pp. 5856–5857, 2008.
[18]
R. Muszynski, B. Seger, and P. V. Kamat, “Decorating graphene sheets with gold nanoparticles,” The Journal of Physical Chemistry C, vol. 112, no. 14, pp. 5263–5266, 2008.
[19]
T. Cassagneau and J. H. Fendler, “High density rechargeable lithium-ion batteries self-assembled from graphite oxide nanoplatelets and polyelectrolytes,” Advanced Materials, vol. 10, no. 11, pp. 877–881, 1998.
[20]
S. Gilje, S. Han, M. Wang, K. L. Wang, and R. B. Kaner, “A chemical route to graphene for device applications,” Nano Letters, vol. 7, no. 11, pp. 3394–3398, 2007.
[21]
F. Schedin, A. K. Geim, S. V. Morozov et al., “Detection of individual gas molecules adsorbed on graphene,” Nature Materials, vol. 6, no. 9, pp. 652–655, 2007.
[22]
C. Shan, H. Yang, J. Song, D. Han, A. Ivaska, and L. I. Niu, “Direct electrochemistry of glucose oxidase and biosensing for glucose based on graphene,” Analytical Chemistry, vol. 81, no. 6, pp. 2378–2382, 2009.
[23]
M. Zhou, Y. Zhai, and S. Dong, “Electrochemical sensing and biosensing platform based on chemically reduced graphene oxide,” Analytical Chemistry, vol. 81, no. 14, pp. 5603–5613, 2009.
[24]
U. Lange, T. Hirsch, V. M. Mirsky, and O. S. Wolfbeis, “Hydrogen sensor based on a graphene-palladium nanocomposite,” Electrochimica Acta, vol. 56, no. 10, pp. 3707–3712, 2011.
[25]
Y. Wang, Y. Shao, D. W. Matson, J. Li, and Y. Lin, “Nitrogen-doped graphene and its application in electrochemical biosensing,” ACS Nano, vol. 4, no. 4, pp. 1790–1798, 2010.
[26]
W. S. Hummers and R. E. Offeman, “Preparation of graphitic oxide,” Journal of the American Chemical Society, vol. 80, no. 6, p. 1339, 1958.
[27]
X. Q. Lin, J. B. He, and Z. G. Zha, “Simultaneous determination of quercetin and rutin at a multi-wall carbon-nanotube paste electrodes by reversing differential pulse voltammetry,” Sensors and Actuators, B: Chemical, vol. 119, no. 2, pp. 608–614, 2006.
[28]
I. R. W. Z. de Oliveira, S. C. Fernandes, and I. C. Vieira, “Development of a biosensor based on gilo peroxidase immobilized on chitosan chemically crosslinked with epichlorohydrin for determination of rutin,” Journal of Pharmaceutical and Biomedical Analysis, vol. 41, no. 2, pp. 366–372, 2006.
[29]
B. Zeng, S. Wei, F. Xiao, and F. Zhao, “Voltammetric behavior and determination of rutin at a single-walled carbon nanotubes modified gold electrode,” Sensors and Actuators, B: Chemical, vol. 115, no. 1, pp. 240–246, 2006.
