Increased expression of NAF1 contributes to malignant phenotypes of glioma cells through promoting protein synthesis and associates with poor patient survival

a The qRT-PCR assay was carried out to assess mRNA expression of NAF1 in normal brain tissues (N, n？=？8) and gliomas (T, n？=？30). β-Actin mRNA levels were used to normalize the NAF1 expression. Data were presented as mean？±？SD. ***P？<？0.001 (n？=？3). b The extent of specific staining (brown color) indicated the expression of NAF1. Upper images are representative images of immunohistochemistry (IHC) on histologic slides of normal controls, low-grade gliomas (LGGs), and glioblastoma (GBMs) using anti-NAF1 antibody. The lower images show magnifications of the area indicated by the black squares. Scale bars, 200？μm. c NAF1 expression was detected by western blot analysis in three cases of normal brain tissues and six cases of glioma patients with GAPDH as a loading control. Shown is representative of three independently preformed western blot experiments (left panels). Shown is the quantitative illustration of the levels of NAF1 proteins using densitometry (right panels). Data were shown as mean？±？SD. ***P？<？0.001 (n？=？3). The relationship of increased expression of NAF1 with the survival of LGG patients (d) and GBM patients (e) in The Cancer Genome Atlas (TCGA) datase