Substance-P Ameliorates Dextran Sodium Sulfate-Induced Intestinal Damage by Preserving Tissue Barrier Function

The effect of SP in DSS-induced intestine damages. Mice received the drinking water with 1.5% DSS for 7 days and SP was administrated to tail vein during the intake of DSS. A Experimental schedule for colitis induction and SP treatment. B Gross view of intestine tissue. C Quantification of colon length (cm). D Histological analysis was performed by H&E staining. E Crypt length was measured as a distance from the bottom to apical crypt using Image J program. F Collagen deposition was carried out using Masson’s trichrome staining. Results are shown as mean values ± SD. n = 10/group, *p < 0.05, **p < 0.01, ***p < 0.001. Scale bar: 50 μ