MiR-214 Regulates the Human Hair Follicle Stem Cell Proliferation and Differentiation by Targeting EZH2 and Wnt/β-Catenin Signaling Way In Vitro

Primary HFSC culture and identification of HFSCs and miR-214 expression during proliferation and differentiation stages. A–F Primary HFSCs were successfully cultured and subcultured. A, B Day 15, HFSCs emerge from the tissue (10？×？10 and 10？×？20); C Day 21, HFSCs emerge from the tissue (10？×？10); D Day 7 after primary culture (scattered HFSCs, 10？×？10); E: 1st generation; F 2nd generation; G Identification of HFSCs by flow cytometry using LGR5 (+), CD200 (++), CD271 (？), and CK15 (？) as markers; H miR-214 expression during differentiation to TA cell