Assessing of Antidiabetic and Ameliorative Effect of Lupin Seed Aqueous Extract on Hyperglycemia, Hyperlipidemia and Effect on pdx1, Nkx6.1, Insulin-1, GLUT-2 and Glucokinase？Genes Expression in Streptozotocin-induced Diabetic Rats

Diabetes mellitus (DM) belong to the major health problems causing mortality and morbidity in the world. Our study aimed to investigate effects of Lupinus Albus (LA) seed extract on streptozotocin (STZ)-induced diabetic rats. Thirty mature Sprague Dawley male rats were used in this study. Three experimental groups were used as follows: GI- Control rats (Normal), GII- Diabetic rats and GIII- Diabetic rats treated with LA aqueous extract (7mg/100g B.wt) by oral gavage. Insulin, Fasting blood glucose (FBG), glycosylated hemoglobin (HbA1c) and serum lipid profile assays of all rats were carried out. Gene expressions of Pancreatic and duodenal homeobox 1 (Pdx-1), homeobox protein (Nkx6.1), insulin-1, liver glucose transporter-2 (GLUT-2) and glucokinase were performed by real-time PCR. Results obtained for diabetic rats orally gavage with LA extract revealed an improvement in insulin with reduction in FBG, HbA1c% and significant improvement in serum lipid profile. Additionally, LA aqueous extract induced a mild increase in expression of biomarkers genes for pancreatic βeta cells (β-cells) function, insulin secretion, liver GLUT-2 and glucokinase when compared to STZ-diabetic rats. This research highlights LA seed extract as a nutraceutical for improving insulin secretion in diabetic rats