Evaluation of Anti-aging Activity of the Biofield Energy Treated Novel Test Formulation Using SIRT1 and Telomerase Activity in in Vitro Model - Evaluation of Anti-aging Activity of the Biofield Energy Treated Novel Test Formulation Using SIRT1 and Telomerase Activity in in Vitro Model - Open Access Pub

Telomerase and SIRT1 (member of the sirtuin protein family) along with the lifestyle and diet are the major determinants of aging and its associated diseases such as cancer and cardiovascular disorders. The study objective was to investigate the effect of Consciousness Energy Healing based novel test formulation in pre-adipocytes (3T3-L1) and human peripheral blood mononuclear cells (PBMCs) for anti-aging activity using SIRT1 and telomerase assay. The test formulation was divided into two parts. One portion was denoted as the untreated test item without any Biofield Energy Treatment, while the other portion was defined as the Biofield Energy Healing Treatment, which received the Biofield Energy Healing Treatment by a renowned Biofield Energy Healer, Mahendra Kumar Trivedi. The cell viability using MTT assay showed that the cell viability of 3T3-L1 and PBMCs cells was more than 70% indicating a safe and nontoxic profile. The experimental data in PBMCs cells showed that the Biofield Energy Treated Test formulation showed a significant improved telomerase activity by 39.25%, 20.86%, and 17.95% at concentrations 0.01, 5, and 100 μg/mL, respectively as compared with the untreated test formulation group. These results indicate that the Biofield Energy Healing Treatment would be the significant approach to prevent aging-related disorders such as decline cardiovascular diseases, osteoporosis, dementia, osteoarthritis, Alzheimer’s, hypertension, cancer, Parkinson's Disease, Chronic Obstructive Pulmonary Disease (COPD), Stress, Asthma, cataract, age-related macular degeneration (AMD), hearing loss and metabolic disorders. DOI10.14302/issn.2474-7785.jarh-19-2994 Aging (a complex process) leads to the detrimental changes at both the molecular and cellular levels, which results in functional decline at the tissue and organ level. However, a factor such as nutrition, stress, injury, diseases and associated environmental factors affects the growth and aging 1. Physiological function has been progressively declined and is associated with dysfunction of body function leads to death. However, aging process can be slow down using scientific efforts, which can extend the lifespan and overall average lifespan. The data from WHO (World Health Organization) suggested that between 2000 and 2050 the world’s population will be doubled over 60 years of age from 11% to 22% 2. Thus, maintain the health lifestyle has been practiced by many countries, which help to prevent and postponing the aging process. Aging can be induced by various intrinsic and extrinsic factors. Alcohol