PhGRP基因RNAi干涉载体转化矮牵牛的初步研究
Transformation of petunia by PhGRP gene RNA interference vector

为鉴定在矮牵牛中筛选到的花药发育早期特异表达基因PhGRP的功能，构建了该基因的RNAi干涉载体，同源转化矮牵牛并进行了表型观测。与野生型对照相比，转基因矮牵牛植株的整体外观形态没有发生明显的变化，但其花器官的大部分形态指标均显著增大；花粉粒染色及离体培养表明其育性（包括活力和萌发力）显著降低；扫描电镜观察发现花粉粒大都畸形，外壁纹饰粗大；半薄切片结果显示，花药绒毡层发达且降解缓慢，推测绒毡层的发育异常为导致花粉粒败育的主要原因。
The RNAi vector of PhGRP was constructed and transformed into petunia to identify the function of PhGRP expressed specifically in the early development of petunia anther.Compared with wild type，no obvious difference of the plant appearance was found in transgenic petunia，but most of the morphological indexes of its floral organs significantly increased.Results of the pollen grain staining and in vitro culture showed that the male fertility of transgenic lines was significantly lower than that of the wild type.Results of scanning electron microscope observation showed pollen grains of the transgenic lines were deformed，with abnormal exine decoration.Results of cytological observation showed that the tapetum developed thickly and degraded slowly in the transgenic lines，indicating that the abnormal development of tapetum might be the main cause of aborted pollen grain.