A procedure for the green synthesis of silver nanoparticles (AgNPs) usingCannabis sativa(hemp plant) as a stabilizing
media was developed and antibacterial activity was tested. Within 30 minutes of
heatingthe mixture
of silver nitrate and hemp extract, the formation of silver nanoparticles took place
under the complete absence of a chemical reducing or an additional stabilizing agent.
The so-formed AgNPs were characterized using different optical spectroscopy and
electron microscopy techniques. The initial formation of AgNPs was established from
UV-Vis data based on surface plasmon resonance (SPR) of AgNPs at ~417 nm. The exact size, shape,and elemental composition of
AgNPs were established from ESEM images and EDS data.The antibacterial activity of these nanoparticles was
studied on Gram-positive Staphylococcus aureus,
and Gram-negative Escherichia colifollowing Disk diffusion and Minimum Inhibitory Concentration (MIC) tests.
Results showed that the biosynthesis of silver nanoparticles using hemp extract could be a simple,inexpensive,
and biocompatible method.
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