It is known to be that lactic acid bacteria induce the IL-12. IL-12 activates NK cells and promotes the production of IFN-γ. IFN-γ activates macrophages, resulting in enhanced phagocytosis and bactericidal activity. We have been investigating fermented foods that activate the immune function. For that purpose, a specific antibody is required. We tried to express IL-12p35 by the usual method, but IL-12p35 was not expressed at all. In the present study, we constructed, purified human IL-12p35 and obtained a specific antibody against IL-12p35. We also purified human IFN-γ and obtained specific antibody against IFN-γ. We have established a method for expressing poorly expressed proteins. The method we have established can be applied to the purification of poorly expressed proteins and antibody production.
References
[1]
Guo, Y.F., Cao, W. and Zhu, Y. (2019) Immunoregulatory Functions of the IL-12 Family of Cytokines in Antiviral Systems. Viruses, 11, 772.
https://doi.org/10.3390/v11090772
[2]
Masuda, T., Kimura, M., Okada, S. and Yasui, H. (2010) Pediococcus pentosaceus Sn26 Inhibits IgE Production and the Occurrence of Ovalbumin-Induced Allergic Diarrhea in Mice. Bioscience, Biotechnology, and Biochemistry, 74, 329-335.
https://doi.org/10.1271/bbb.90656
[3]
Laemmli, U.K. (1970) Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4. Nature, 227, 680-685. https://doi.org/10.1038/227680a0
[4]
Towbin, H., Staechelin, T. and Gordon, J. (1979) Electrophoretic Transfer of Proteins from Polyacrylamide Gels to Nitrocellulose Sheets: Procedure and Some Applications. Proceedings of the National Academy of Sciences of the United States of America, 76, 4350-4354. https://doi.org/10.1073/pnas.76.9.4350
[5]
Uemura, S., Nakajima, Y., Yoshida, Y., Furuya, M., Matsutani, S., Kawate, S., Ikeda, S.I., Tsuji, N., Grave, E., Wakui, H. and Itoh, H. (2020) Biochemical Properties of Human Full-Length Aryl Hydrocarbon Receptor (AhR). The Journal of Biochemistry, 168, 285-294. https://doi.org/10.1093/jb/mvaa047
