Antioxidant Evaluation of Romasanjanana Lepa- A Compound Formulation for Indralupta

Alopecia Areata (AA) is the common form of hair loss affecting the quality of life of many patients. In Ayurved, loss of hair is coined as ‘Indralupta’ described in Kshudra Rogas. Vata Pitta Kapha Dosha along with the vitiation of Rakta is responsible for pathophysiology of Indralupta whereas, according to modern science, Alopecia areata is a tissue-restricted autoimmune disease. Therefore it is important to evaluate the role of antioxidant in the treatment. Ayurveda has advocated Lepa in Indralupta. Therefore a compound formulation consisting of leaf of Karanja, fruit of Kapittha, Kasisa and Hastidant Mashi was formulated following standard guideline. To evaluate the antioxidant activity in the compound formulation, it was subjected to Total phenolic content by Folin’s reagent, Antioxidant by DPPH(1,1-diphenyl-2-picrylhydrazyl), Antioxidant by FRAP (ferric reducing antioxidant power), total flavonoid content, and assessment of phenolic compound by HPLC method on distilled water, methanolic and ethanolic extract of the formulation. Result of the study showed maximum Total phenolic content in distilled water extract, whereas total flavonoid was maximum in methanolic extract, Antioxidant by DPPH was maximum (85.24±0.30) in distilled water extract. Whereas by FRAP method, it was maximum (0.017±0.02) in methanolic extract. Total phenolic content was highest in ethanolic extract.