Resistance to β-Lactamines by Gram Negative Bacteria, Producing Several Types of Enzymes, Isolated from Urines in Pediatric Center of Ouagadougou in Burkina Faso

Extended Spectrum β-Lactamases (ESBL) producing bacteria have been reported in several countries, but there is little information available on the different types of ESBL produced by Enterobacteria in Burkina Faso. For compensate for the lack of scientific information of extreme importance, this study deals with molecular characterization of several types of extended spectrum β-lactamases (ESBL) produced by Enterobacteria strains isolated in pediatric medium. Enterobacteria strains producing ESBL were identified by their profile of resistance to the cephalosporin of third generation (C3G) on Petri box. These strains consist of several bacterial species resistant to antibiotics. The production of ESBL by these strains was confirmed by kinetic approach. The genomes of the different strains were obtained by DNAZOL extraction. The molecular characterization of the ESBL was based on classical PCR and sequencing using specific primers of the CTX-M, SHV and TEM genes. CTX-M, SHV and TEM genes were searched in DNAs extracted from 30 isolates positives to ESBL production. CTX-M-15 was found in the 30 isolates, CTX-M-15 and SHV-1,-11,-12,-28,-32,-38,-76 or-99 in 11 isolates, CTX-M-15 and TEM-1 in 11 isolates , and CTX-M-15, TEM-1 and SHV-1,-11,-12,-32,- 76 or -99 in 5 isolates. β-lactamases types of CTX-M-15, TEM-1 and SHV-1,-11,-12,-28,-32,-38,-76 and-99 were highlighted in the University Hospital center Pediatric Charles De Gaulle of Ouagadougou/BF