BAX Activation: Mutations Near Its Proposed Non-canonical BH3 Binding Site Reveal Allosteric Changes Controlling Mitochondrial Association

？ BH3 binding to BAX groove essential for late but not early steps in BAX activation ？ Mutations identified in BAX helices 1 and 6 near a proposed second BH3 binding site ？ They reduced BH3 binding to this site and stabilized BAX in inactive conformations ？ They revealed allosteric changes controlling BAX mitochondrial membrane associatio