Human and Extracellular DNA Depletion for Metagenomic Analysis of Complex Clinical Infection Samples Yields Optimized Viable Microbiome Profiles

？ Human and extracellular bacterial DNA can bias metagenomic sequencing ？ Hypotonic lysis, endonuclease digestion limit human and extracellular bacterial DNA ？ Reducing extracellular DNA optimizes metagenomic sequencing of viable bacterial cells ？ Increased microbial sequencing coverage improves detection of important gene