Dissecting N-Glycosylation Dynamics in Chinese Hamster Ovary Cells Fed-batch Cultures using Time Course Omics Analyses

？ Major glycosylated species exhibit temporal dynamics during fed-batch processes ？ Key metabolic pathways linked to N-glycosylation exhibit significant temporal dynamics ？ Dynamics in nucleotide sugar donors (NSDs) directly influences glycoform heterogeneity ？ Glycoform heterogeneity can be mitigated by supplementing NSD biosynthetic precursor