Pilot Scale Extraction of Orthosiphon stamineus Raw Material as Rosmarinic Acid Extract

Abstract A scale-up protocol for rosmarinic acid extraction from Orthosiphon stamineus was developed using the concept of constant power dissipation for a similar kinetic profile. Rosmarinic acid is the bioactive compound in the herb and therefore, large scale extraction for that compound is important for commercial application. The first-order kinetic equation which was established in the preparative scale extraction was used to describe the extraction of rosmarinic acid from the herb. The agitation speed of the pilot large-scale extractor (451 rpm) was determined based on the constant power dissipation to meet the minimal required speed, namely just-suspended speed ( Njs), 450.84 ± 0.88 rpm. The experimental data was fitted well to the proposed kinetic equation with a high correlation coefficient, R 2 0.88, and a low root mean square error, RMSE 3.85. This indicates the good performance of the scale-up approach based on the dynamic criterion. Therefore, the use of constant power dissipation to obtain a similar kinetic profile could be applied for phytochemical extraction from herbal plants. View Full-Tex