Photo- and Aromatic Stacking-Induced Green Emissive Peptidyl Nanoparticles for Cell Imaging and Monitoring of Nucleic Acid Delivery

Owing to their potential applications in biomedicine and biotechnology, peptide nanostructures that exhibit stable intrinsic fluorescence in the visible range are highly desired. This research proposes a facile strategy to construct peptidyl virus-like nanoparticles (NVPs) that show green luminescence by coassembly of two bioactive ferrocene-diphenylalanine-based (Fc-FF) peptides. The green fluorescence of NVPs was originated from the highly ordered structures assembled by the amphiphilic Fc-FF-based peptides via strong π–π stacking interactions. In the assemblies, Fc-FF chromophore can be hydrolyzed under the natural light irradiation, which eliminates the fluorophore quenching effect of Fc and increases the aromatic stacking interactions, thereby giving rise to strong fluorescent nanoparticles. The NVPs could cross cytomembrane barriers by virtue of the HIV V3 peptide and the nuclear localization signal, and could thus be used for long-term cell imaging with excellent photostability and biocompatibility in physiological condition. In addition, NVPs could package DNA and be used to monitor the delivery of DNA, indicating great potential in the tracking and monitoring of genetic biological processes