Circular Environmental Policies in The Industrial Production

In this study, 50 Pseudomonas spp. have been isolated from the instances of waste water purification pool. 27 Pseudomonas aeruginosa and 23 Pseudomonas stutzeri strains were detected according to colony morphologies, Gram staining and type definitions that have been made in the vitek 2 compact 30 (biomerieux) device. Identified strains were exposed by 10 ppm Cr(VI), Cd(II), Cu(II) and Mn(II) concentrations and detected their viabilities against these metals and by this way determined the most resistant strains for each metal. Also, four different concentrations of these metals were determined according to viability of strains. % death values of strains were determined by treated with metal concentrations and LC50 values of metals were calculated. Strains selected for each metal by exposing them to the specified concentrations, EPS isolation has been carried out. In Cr(VI) concentration it has been found that the Pseudomonas stutzeri of the highest EPS production is S46 strains (74.68 mg/L) while the lowest Pseudomonas stutzeri is S18 (39.37 mg/L). In Cd(II) concentration; the Pseudomonas stutzeri of the highest EPS production is S23 (60.93 mg/L), while the lowest EPS production is Pseudomonas aeruginosa S10 strains (40.93 mg/L). In Cu(II) concentration, the Pseudomonas stutzeri of the highest EPS production is S45 strains (76.06 mg/L) while the lowest EPS production is Pseudomonas aeruginosa S8 strains (28.28 mg/L). In Mn (II) concentration, the Pseudomonas stutzeri of the highest EPS production is S44 strains (46.40 mg/L), while the Pseudomonas stutzeri of the lowest EPS production is S50 strains (26.56 mg/L). As a result, exposed to increasing metal dose of Pseudomonas spp., the amount of EPS produced by the strains has been found to be increased. By this study it has been expressed the effect of some heavy metals of Pseudomonas spp strains, which have been isolated from wastewater, to the EPS production in terms of biotechnology