Determination of Acetylsalicylic Acid, Paracetamol and Caffeine in Drug Formulation with Chemometric Methods

In this study, the results of ultraviolet spectrophotometry of paracetamol, acetylsalicylic acid and caffeine active substances in tablet drug sample were evaluated together with chemometric methods. In the experimental study, the synthetic mixtures were prepared by taking each drug from the active ingredient after obtaining the individual pure spectra of the substances. At the next stage, a tablet sample analysis was performed. From the chemometric methods, the principle component regression method (PCR) and the partial least squares method (PLS) have been successfully applied in the quantification of the synthetic mixtures and the tablet sample. When the data obtained are statistically analyzed, both the calculated recovery values are high and the standard deviations are small enough. In addition, F-test was applied in terms of the suitability of the method and the results were found to be compatible. The results obtained with the help of applied chemometric methods are very fast, simple and reliable results