The Effect of Cholelithiasis and Cholecystectomy on Thiol/Disulfide Homeostasis in Patients Undergoing Cholecystectomy

Objective: The aim of this prospective controlled clinical research study is to investigate the effects of noncomplicated cholelithiasis and laparoscopic cholecystectomy surgery for cholelithiasis in adults on thiol/disulfide balance. Material and Method: The study included healthy volunteers aged 18-80 years (Control group, n=30) and patients with symptomatic gallstones between 18-80 years of age who underwent laparoscopic cholecystectomy (KLS group, n=30). In this study, 2 mL venous blood samples were taken from healthy volunteers for only one time, 1 hour before the operation and 24 hours after the operation from all patients, and thiol/disulfide levels in the serum were examined. Results: At the end of the study, Native Thiol, Total Thiol and Disulfide values of the control group were higher than the preoperative values of the KLS group (p<0.001). The postoperative values of Native Thiol, Total Thiol and Disulfide in the KLS group was found to be lower than the control group (p<0.001). Also in the control group, Disulfide/Native Thiol and Disulfide/Total Thiol ratios were higher than the postoperative values of KLS group and Native Thiol/Total Thiol ratio was measured lower. On the otherhand, there was no difference between the preoperative and postoperative laboratory values of the KLS group. Conclusion: These results suggest that both the pre-operative and post-operative native thiol, total thiol, and disulfide levels were lower in the cholelithiasis patient group because they were consumed in these patients due to the oxidative stres caused by cholelithiasis. On the otherhand, these changes in oxidative metabolism in these patients were thought to provoke stone formation in gallbladder