Reproduction of Ficus pumila L. (Climbing Fig) with Tissue Culture

ABSTRACT: Ficus pumila L., which provides a green wall appearance with its very close texture, is not commonly grown in Turkey despite its significance. Among the studies published so far, there is no record of successful micropropagation of Ficus pumila species. Thus, the objective of the present study was to propagate Ficus pumila with tissue culture, and to expand the use of the species in Turkey. Various growth regulators and doses (IAA, BA, BA + Kinetin) were tested in studies where Murashige and Skoog (MS) compounds are used commonly as nutrient media. Plant leafs and leaf stems were used as explant. It was possible to obtain plants with different explant and nutrient media combinations. Cultures were kept in climate chamber at 23 ° C temperature and 70% humidity and in 16 hours light and 8 hours dark photoperiodic conditions. In the present study, it was observed that the combination of BA dose and cytokinin in BA and MS were successful in the findings. The highest shoot formation was obtained in MS medium supplemented with 1 mg / l BA doses and combinations