The Effect on Antioxidant Defense System of Chondroitin-4-Sulphate in Human Erythrocytes Subject to High Glucose Levels: In The Laboratory Conditions

The purpose of this research was to evaluation the effects on antioxidant defense system of chondroitin-4-sulphate (C4S) in high glucose treated human erythrocytes. 10-20 ml venous blood samples from ten healthy volunteers ages 20-40, nonsmoker, were accumulated in ethylenediamine tetraacetic acid (EDTA) vials for the preparation of red blood cell (RBC) suspensions. Blood for in vitro treatments was divided into six groups. The activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), acetylcholinesterase (AChE), the levels of glutathione (GSH) and malondialdehyde (MDA) were calculated to determine the effects on antioxidant defense system in all groups. As a result of the statistical evaluations, the significant increases (p<0.001) in GSH-Px and AChE enzyme activities were observed in the groups in which C4S applied compared to the groups in which glucose alone applied. At the same time, significant decrease (p<0.001) in MDA levels was obtained in the groups in which C4S applied compared to the groups in which glucose alone applied. These acquired findings have shown that in the erythrocytes to which high amount of glucose has been treated; C4S may have a positive impact upon antioxidant defense systems. As the results of this study, C4S can be said to be benefited as an antioxidant