DEVELOPMENT OF REFLECTOMETRIC INTERFERENCE SPECTROSCOPY BASED SENSOR FOR PARAOKSON DETERMINATION

In this study, a reflectometric interference spectroscopy (RIfS) based sensor was developed that selectively recognizes and binds the paraoxon molecule. For this purpose, first the activation of the CMD (Carboxymethyldextran) chip used in the refractometric interference spectroscopy was performed. 3-Dimethylaminopropyl-N'-ethylcarbodiimide (EDC) and N-hydroxysuccinimide (NHS) were used in the activation process. After activating the chip surface, the AChE (Acetylcholinesterase) enzyme was transferred to the immobilization step. Afterwards the AChE enzyme immobilization was performed on the chip surface, analysis of paraoxon solutions in different concentrations was performed. For the calibration line, a close correlation was observed in the refractometric interfering spectroscopy up to 0,01-150 ppm, the highest concentration of paraoxon used. The LOD value was calculated as 9,9x10-8 M and the LOQ value was calculated as 1,97x10-7 M