Objective: Based on the GEO database, the differential genes of sinusoidal endothelial cells in cirrhotic rats were analyzed. Methods: In the GEO database, the differential gene expressions of liver sinusoidal endothelial cells in cirrhotic rats were obtained. The screening was performed according to P < 0.01 and differential multiple factor ≥ 4. The obtained genes were input into the DAVID database for enrichment analysis of genes and pathways. The GeneMania and string databases were then used for protein interaction analysis. Results: The GSE1843 dataset was obtained in the GEO database, and three pathways significantly associated with cirrhosis and 13 differential genes enriched in three pathways were screened. Text mining revealed that 11 differential genes were directly associated with cirrhosis. The other two were indirectly linked by other genes. The screened genes and known gene formation networks were discovered by the GeneMania tool. Conclusion: CDH2 and COL1A1 may be important target genes for cirrhosis.
References
[1]
Lu, F.M. and Zhuang, H. (2009) Management of Hepatitis B in China. Chinese Medical Journal, 122, 3-4.
[2]
Wang, F.S., Fan, J.G., Zhang, Z., et al. (2014) The Global Burden of Liver Disease: The Major Impact of China. Hepatology, 60, 2099-2108. https://doi.org/10.1002/hep.27406
[3]
Lozano, R., Naghavi, M., Foreman, K., et al. (2012) Global and Regional Mortality from 235 Causes of Death for 20 Age Groups in 1990 and 2010: A Systematic Analysis for the Global Burden of Disease Study 2010. The Lancet, 380, 2095-2128. https://doi.org/10.1016/S0140-6736(12)61728-0
[4]
Knolle, P.A., Bottcher, J. and Huang, L.R. (2015) The Role of Hepatic Immune Regulation Systemic Immunity to Viral Infection. Medical Microbiology and Immunology, 204, 21-27. https://doi.org/10.1007/s00430-014-0371-0
[5]
Li, S.M., Li, G.Y., Peng, Y. and Zhao, T.J. (2016) Effects of Hepatic Sinusoidal Endothelial Cells on Hepatic Fibrosis and Its Regulatory Mechanism. Journal of Chongqing Medical University, 41, 624-627.
[6]
Hugues, S., Morales-Ruiz, M., Fernandez-Varo, G., Ros, J., et al. (2005) Microarray Analysis of Endothelial Differentially Expressed Genes in the Liver of Cirrhotic Rats. Gastroenterology, 129, 1686-1695. https://doi.org/10.1053/j.gastro.2005.09.006
[7]
Barrett, T., Wilhite, S.E., Ledoux, P., et al. (2013) NCBI GEO: Archive for Functional Genomics Data Sets-Update. Nucleic Acids Research, 41, D991-D995. https://doi.org/10.1093/nar/gks1193
[8]
Huang da, W., Sherman, B.T. and Lempicki, R.A. (2009) Bioinformatics Enrichment Tools: Paths toward the Comprehensive Functional Analysis of Large Gene Lists. Nucleic Acids Research, 37, 1-13. https://doi.org/10.1093/nar/gkn923
[9]
Dennis, G., Sherman, B.T., Hosack, D.A., et al. (2003) DAVID: Database for Annotation, Visualization, and Integrated Discovery. Genome Biology, 4, P3. https://doi.org/10.1186/gb-2003-4-5-p3
[10]
Friedman, S.L. (2005) Liver Fibrosis: New Frontiers in Liver Disease (English). Compilation of Papers of the First International Conference on Liver Disease of Integrated Traditional Chinese and Western Medicine, Shanghai, 8-10.
[11]
Wang, J.H., Wei, X.F., Zhang, C.S., et al. (2012) Expression and Significance of Collagen Gene in the Liver of Mice in the Middle Stage of Metacercariae Infection. Journal of Pathogen Biology, 7, 653-656.
[12]
Fu, Y., Dan, Z.-L., Tang, W.-X. and Yan, W. (2006) Effect of All-Trans Retinoic Acid on Expression of TGF-β1, CTGF and Col1a1 in Chronic Alcoholic Liver Disease in Rats. World Chinese Journal of Digestology, 14, 179-183.
[13]
Davis, B.H., Kramer, R.T. and Davidson, N.O. (1990) Retinoic Acid Modulates Rat Ito Cell Proliferation, Collagen, and Transforming Growth Factor-Beta Production. Journal of Clinical Investigation, 86, 2062-2070. https://doi.org/10.1172/JCI114943
[14]
Baumgart, E., Cohen, M.S., Silva Neto, B., et al. (2007) Identification and Prognostic Significance of an Epithelial-Mesenchymal Transition Expression Profile in Human Bladder Tumors. Clinical Cancer Research, 13, 1685-1694. https://doi.org/10.1158/1078-0432.CCR-06-2330
[15]
Cao, G.X., Zhang, Y.X. and Wang, X.L. (2014) Expression of Genes Related to Epithelial-Stromal Differentiation in Bladder Cancer. Jiangsu Medical Journal, 40, 2061-2062.
[16]
Huber, M.A., Kraut, N. and Beug, H. (2005) Molecular Requirements for Epithelial-Mesenchymal Transition during Tumor Progression. Current Opinion in Cell Biology, 17, 548-558. https://doi.org/10.1016/j.ceb.2005.08.001
