Microbial fuel cell (MFC) is
one of renewable biofuel production technology that directly converts biomass
to electricity. Cellulosic biomass is particularly attractive renewable
resources for its low cost and abundance and neutral carbon balance. However,
methanogenesis remains as a major factor limiting MFC performance. The current
study reports that saponin addition at 0.05% w/v dose to anolyte in MFCs
inhibited methanogenesis and improves power generation and cellulose
fermentation. Mediator-less two chamber H-type MFCs were prepared using rumen fluid as anode inocula at 20% v/v of
anolyte to convert finely ground pine tree (Avicel) at 2%, w/v to electricity.
Saponin was added to the anode of MFC at 0.005% or 0.05% v/v dosage for
treatment. MFC power and current across an external resistor were
measured daily for 10d. On d10, collected gases from anode compartment were
measured for total gas volume and analyzed for gas composition on gas
chromatography. Supplementation of saponin to MFC at 0.005% did not have any
effects on electricity generation or biogas production and composition. Saponin
at 0.05% dose reduced 10% of methane production and increased 40% of CO2 production and 6.4% of total gas production for 10d MFC operation. Voltage
across resistor prior to treatment addition (d0) was 164.75 ± 9.07 mV. In
control group, voltage across
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