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阴道保健潜力的乳酸菌的分离、鉴定与筛选
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Abstract:
本研究旨在分离和筛选具有潜在女性私密护理作用的乳酸菌(LAB)。收集女性一次性卫生用品上的残留物作为标本，通过革兰氏染色确认阴道菌群正常。用LAB选择性培养基MRS培养从标本中分离出单个LAB菌落，并通过16s DNA鉴定物种。进一步对这些分离物进行了多项功能筛选试验，包括抗菌能力、H2O2产生试验和宫颈细胞粘附试验。结果表明L. gasseri GKA2对致病菌大肠杆菌(E. coli)和肺炎克雷伯菌(K. pneumonia)具有抑制作用。L. gasseri GKA2和L. crispatus GKJ6都具有产生H2O2的能力。在宫颈细胞粘附试验中，菌株GKA2和GKJ6的粘附能力分别为92.21%和92.46%。综上所述，乳酸杆菌GKA2和GKJ6具有阴道保健的潜力。未来可对这两种菌株进行体内试验验证，作为贴身保健品的应用进一步验证定量生产的可行性。
This study was to isolate and screen the lactic acid bacteria (LAB) with potential female intimate care. The residues on the women’s disposal sanitary were collected as specimens and confirmed the normal vaginal bacteria flora by Gram staining. Single LAB colonies were isolated from the specimens by culturing with LAB selective medium MRS and identified the species by 16s DNA. Several functional screening tests for these isolations were further carried out, including antibacterial ability, H2O2 production test, and cervical cell adhesion test. Results showed that L. gasseri GKA2 could inhibit pathogenic bacteria E. coli and K. pneumonia. Both L. gasseri GKA2 and L. crispatus GKJ6 had abilities to produce H2O2. In the cervical cell adhesion test, strains GKA2 and GKJ6 had 92.21% and 92.46% adhesion ability, respectively. To summarize, Lactobacillus GKA2 and GKJ6 have the potential for vaginal health care. In the future, these two strains can be tested in vivo for verification, and the feasibility of quantitative production can be further tested as the application of intimate health care products.
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