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不同固定液对海马卵巢冰冻切片HE染色质量的影响
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Abstract:
海马是世界上极其短缺的珍贵药源性海洋硬骨鱼类，具有独特的雄性育儿机制和性腺结构。解析海马性腺的组织学结构对提高海马人工养殖的效率具有重要意义。冰冻组织切片相比石蜡切片具有快捷、操作简便等优点，然高质量冰冻切片的制片易受多重因素影响，其中固定液是影响后续HE染色的关键因素之一。本实验比较了8种常用固定液对海马卵巢冰冻切片HE染色的影响。结果显示，冰醋酸固定的卵巢组织结构清晰、细胞核边界分明，核质比高，且卵黄颗粒亦着色明显，是一种较为理想的海马卵巢冰冻切片固定液。
Seahorse is a kind of precious marine medicinal teleosts with special father’s rearing pattern and gonadal structure. Analyzing the histological structure of seahorse gonads is significant of improvement in efficiency of artificial breeding. Compared with paraffin section, frozen section has a fast and simple operation, while the quality of which is susceptible to multiple factors. The fixative is a key factor for Hematoxylin-Eosin (HE) staining. In our study, we chose 8 kinds of fixatives for the frozen section of seahorse ovary and compared the quality of HE stain, results showed that glacial acetic acid fixation was an ideal fixative for frozen sections of seahorse ovary stained with HE, which displayed a clear tissue structure with distinct nuclear boundary, high nucleo-plasmic contrast and bright-coloured yolk granules.
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