Background: The emergence of carbapenemase producingAcinetobacter baumanniiis increasingly reported nowadays and constitutes a
major problem to the intensive care unit (ICU) patients with notable
extensive-drug resistance ability. The study investigates carbapenemase
producing A. baumannii strains exhibiting an extensively drug-resistant
(XDR) phenotype, isolated from ICU patients in Khartoum. Methods: A
total of 100 nonduplicate Gram-negative coccobacilli strains were obtained from
microbiology laboratory of ICU patients’ clinical isolates. Molecular identification of A. baumannii was performed by targeting 16S rRNA gene using specifically designed
primers. Then, XDR strains were determined by susceptibility testing (disc
diffusion). For detection of carbapenemase genes Polymerase chain reaction
(PCR) was carried out.Result: Of 100 ICU clinical isolates, 38 (38.0%) was confirmed A. baumannii strains, those strains showed 100% carbapenem resistance and 60.5% extensive
drug resistance to the antibiotics tested. The frequency of carbapenemase
producer was 57.9% (22/38) of carbapenem resistance A. baumannii (CRAB). The most common carbapenemase associated with
resistance was blaOXA gene followed by blaNDM and blaGES
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