|
|
MMP11基因对卵巢癌细胞增殖能力的影响
|
Abstract:
目的：分析MMP11基因在卵巢癌细胞中的表达特征及对细胞增殖的影响。方法：运用实时荧光定量PCR (qRT-PCR)和Western blotting分别在RNA和蛋白质水平上检测卵巢癌临床样本和卵巢癌细胞系(COV504)中MMP11基因的表达，并用CCK8实验研究MMP11对细胞增殖能力的影响。结果：在卵巢癌临床样本和细胞系中，MMP11基因的表达明显上调，且对卵巢癌细胞的增殖有促进作用。结论：MMP11与卵巢癌起始和发展存在重要关系，且有可能成为卵巢癌的诊断和治疗相关的有效的生物标记物。
Objective: To define the MMP11 expression characteristics and analyse the effect of MMP11 on the proliferation of ovarian cancer cells. Method: qRT-PCR and Western blotting were performed to analyse MMP11 expression level in clinical tissues and cell lines of ovarian cancer (COV504), either on RNA level, or on protein level. Additionally, CCK8 assay was validated to assess the influence of MMP11 on the proliferation of ovarian cancer cells. Result: The expression of MMP11 was up-regulated in ovarian cancer and MMP11 could promote the proliferation of ovarian cancer cells. Conclusion: MMP11 was positively associated with the initiation and progression of ovarian cancer, subsequently rendering it as potentially effective biomarker for the early diagnosis and treatment of ovarian cancer.
| [1] | Yu, H.L., Ma, X.D., Tong, J.F., et al. (2019) WTAP Is a Prognostic Marker of High-Grade Serous Ovarian Cancer and Regulates the Progression of Ovarian Cancer Cells. OncoTargets and Therapy, 12, 6191-6201.
https://doi.org/10.2147/OTT.S205730 |
| [2] | Hua, W., Zhao, Y., Jin, X., et al. (2018) METTL3 Promotes Ovarian Carcinoma Growth and Invasion through the Regulation of AXL Translation and Epithelial to Mesenchymal Transition. Gynecologic Oncology, 151, 356-365.
https://doi.org/10.1016/j.ygyno.2018.09.015 |
| [3] | Pickup, M.W., Mouw, J.K. and Weaver, V.M. (2014) The Extracellular Matrix Modulates the Hallmarks of Cancer. EMBO Reports, 15, 1243-1253. https://doi.org/10.15252/embr.201439246 |
| [4] | Pittayapruek, P., Meephansan, J., Prapapan, O., et al. (2016) Role of Matrix Metalloproteinases in Photoaging and Photocarcinogenesis. International Journal of Molecular Sciences, 17, 868. https://doi.org/10.3390/ijms17060868 |
| [5] | Akgül, B., Pfefferle, R., Marcuzzi, G.P., et al. (2006) Expression of Matrix Metalloproteinase (MMP)-2, MMP-9, MMP-13, and MT1-MMP in Skin Tumors of Human Papillomavirus Type 8 Transgenic Mice. Experimental Dermatology, 15, 35-42. https://doi.org/10.1111/j.0906-6705.2005.00387.x |
| [6] | Bassiouni, W., Ali, M.A.M. and Schulz, R. (2021) Multifunctional Intracellular Matrix Metalloproteinases: Implications in Disease. The FEBS Journal, 288, 7162-7182. https://doi.org/10.1111/febs.15701 |
| [7] | Murph, Y.G. and Nagase, H. (2008) Progress in Matrix Metalloproteinase Research. Molecular Aspects of Medicine, 29, 290-308. https://doi.org/10.1016/j.mam.2008.05.002 |
| [8] | Quintero-Fabián, S., Arreola, R., Becerril-Villanueva, E., et al. (2019) Role of Matrix Metalloproteinases in Angiogenesis and Cancer. Frontiers in Oncology, 9, Article No. 1370. https://doi.org/10.3389/fonc.2019.01370 |
| [9] | Huang, H., (2018) Matrix Metalloproteinase-9 (MMP-9) as a Cancer Biomarker and MMP-9 Biosensors: Recent Advances. Sensors, 18, 3249. https://doi.org/10.3390/s18103249 |
| [10] | Gobin, E., Bagwell, K., Wagner, J., et al. (2019) A Pan-Cancer Perspective of Matrix Metalloproteases (MMP) Gene Expression Profile and Their Diagnostic/Prognostic Potential. BMC Cancer, 19, Article No. 581.
https://doi.org/10.1186/s12885-019-5768-0 |
| [11] | Zhang, X., Huang, S., Guo, J., et al. (2016) Insights into the Distinct Roles of MMP-11 in Tumor Biology and Future Therapeutics. International Journal of Oncology, 48, 1783-1793. https://doi.org/10.3892/ijo.2016.3400 |
| [12] | Wang, Y., Wei, Y., Fan, X., et al. (2020) MicroRNA-125b as a Tumor Suppressor by Targeting MMP11 in Breast Cancer. Thoracic Cancer, 11, 1613-1620. https://doi.org/10.1111/1759-7714.13441 |
| [13] | Eiro, N., Cid, S., Fernández, B., et al. (2019) MMP11 Expression in Intratumoral Inflammatory Cells in Breast Cancer. Histopathology, 75, 916-930. https://doi.org/10.1111/his.13956 |
| [14] | Xu, G., Zhang, B., Ye, J., et al. (2019) Exosomal miRNA-139 in Cancer-Associated Fibroblasts Inhibits Gastric Cancer Progression by Repressing MMP11 Expression. International Journal of Biological Sciences, 15, 2320-2329.
https://doi.org/10.7150/ijbs.33750 |
| [15] | Peruzzi, D., Mori, F., Conforti, A., et al. (2009) MMP11: A Novel Target Antigen for Cancer Immunotherapy. Clinical Cancer Research, 15, 4104-4113. https://doi.org/10.1158/1078-0432.CCR-08-3226 |
