一株分离自猪粪堆肥细菌的生物学特性研究
Study on the Biological Characteristics of a Bacterial Strain Isolated from Gig Manure Compost

目的：从高寒地区猪粪堆肥中筛选得到一株编号为DF200620的细菌，对其进行生物学特性的研究。方法：通过细菌培养，革兰氏染色和比浊法等方法对菌株DF200620的形态，培养特征，遗传型特征，运动性与需氧性，以及与环境因子(温度，pH，盐度，水分)的关系进行了研究。结果：结果表明，菌株DF200620为革兰氏阳性的杆状细菌，菌落为圆形，不透明，表面光滑，淡黄色，分子鉴定结果确定菌株DF200620为赖氨酸芽孢杆菌。菌株生长曲线在0~2 h为调整期，2~8 h内为对数期，8~16 h为稳定期，16 h以后为衰亡期。菌株生长的最适温度为35℃，最适pH为7，在NaCl浓度范围为0%~7% (W/V)，聚乙二醇浓度范围为5~25 g/100mL内能正常生长。结论：此研究结果可为赖氨酸芽孢杆菌的开发利用奠定基础，同时为丰富堆肥中有益微生物的菌株资源、以及为当地农业废弃物的高效利用提供依据。
Objective: To study the biological characteristics of a bacterial strain named DF200620, screened from pig manure compost in alpine region. Methods: In this study, the morphology, culture characteristics, genotypic characteristics, motility and aerobic of strain DF200620, as well as the relationships with environmental factors including temperature, pH, salinity and moisture were studied by bacterial culture, gram staining and turbidimetry. Results: The results showed that strain DF200620 is gram-positive and rod shaped. Colonies are round, opaque, smooth and pale yellow. The growth curve of the strain was adjusted from 0 to 2 h; the logarithmic phase occurred within 2~8 h; 8~16 h is stable period; after 16 h, the decline stage was observed. The optimum temperature was 35？C, the optimum pH was 7, and the strain could grow normally in the range of NaCl concentration 0%~7% (W/V) and polyethylene glycol concentration 5~25 g/100mL. Conclusion: Based on these results, it can provide experimental basis and theoretical reference for the development and utilization of Lysinibacillus fusiformis.