This work presents the synthesis and characterization of compounds
derived from the ruthenium transition metal with the nitrogenous ligand
4-aminopy- ridine (4-ampy). The
synthesized compounds were characterized by FTIRmed spectroscopy and TG-DTA thermal analysis. For the
cytotoxic evaluation of ruthenium compounds, a 66.0 μM aqueous solution
containing the complex and the study of data observed in the biological
assessment was performed using variance (ANOVA) analysis, followed by Tukey’s
multiple comparisons test. Differences between treatments were considered
significant when the p-value was less than 0.05 (p < 0.05). TG/DSC thermal
analysis for the first complex suggests a
stoichiometry of [Ru(Cl)3(4-ampy)(H2O)2]·1/2H2O,
which, due to the low solubility in an aqueous medium, was modified to
increase its solubility for biological tests. The analysis of the spectra in
the medium infrared region (FTIR) for the complex [Ru(Cl)3(4-ampy)(H2O)2]·1/2H2O,
shows displacements of the bands observed at 1625-1566 cm﹣1ν(C=C) e (C=N), indicating that coordination to the
References
[1]
Allardyce, C.S. and Dyson, P.J. (2001) Ruthenium in Medicine: Current Clinical Uses and Future Prospects. Platinum Metals Reviews, 45, 62-69.
[2]
AlQaradawi, S.Y. and Nour, E.M. (2006) Synthesis and Spectroscopic Structural Studies of the Adducts Formed in the Reaction of Aminopyridines with TCNQ. Journal of Molecular Structure, 794, 251-254.
https://doi.org/10.1016/j.molstruc.2006.02.031
[3]
Buyukmurat, Y. and Akyuz, S. (2003) Theoretical and Experimental Studies of IR Spectra of 4-Aminopyridine Metal(II) Complexes. Journal of Molecular Structure, 651-653, 533-539. https://doi.org/10.1016/S0022-2860(02)00674-9
[4]
Chagas, M.A.S., Galvão, A.D., de Moraes, F.T., et al. (2017) Synthesis, Characterization and Analysis of Leishmanicide Ability of the Compound [Ru (Cl)3(H2O)2(gly)]. 7, 89-101. https://doi.org/10.4236/ojic.2017.74006
[5]
Chandra, S.P. and Singh, A. (2014) Kinetics of Copper (II) Perchlorate Complex with 4-Aminopyridine. Energy and Environment Focus, 3, 202-205.
https://doi.org/10.1166/eef.2014.1114
[6]
Clarke, M. (1989) Symposium on Ruthenium and Other Non-Platinum Metal Complexes in Cancer Chemotherapy. Progress in Clinical Biochemistry and Medicine, 10, 223.
[7]
de Almeida, S.M.V., de Alcantara, F.F., de Brito, C.G.X., et al. (2014) Compostos coordenados híbridos de platina no tratamento do cancer. Revista de Ciências Farmacêuticas Básica e Aplicada, 35, 337-345.
[8]
Frasca, D.R., Gehrig, L.E. and Clarke, M.J. (2001) Cellular Effects of Transferrin Coordinated to [Cl(NH3)5Ru]Cl2 and cis-[Cl2(NH3)4Ru]Cl. Journal of Inorganic Biochemistry, 83, 139-149. https://doi.org/10.1016/S0162-0134(00)00180-X
[9]
Gagliardi, R., Sava, G., Pacor, S., et al. (1994) Antimetastatic Action and Toxicity on Healthy Tissues of Na[trans-RuCl4(DMSO)Im] in the Mouse. Clinical & Experimental Metastasis, 12, 93-100. https://doi.org/10.1007/BF01753975
[10]
Galvão, A.D., de Moraes, F.T., de Sousa, C.C., et al. (2019) Synthesis and Characterization of a New Compound of Cobalt II with Isonicotinamide and Evaluation of the Bactericidal Potential. Open Journal of Inorganic Chemistry, 9, 11-22.
https://doi.org/10.4236/ojic.2019.92002
[11]
Jirsova, K., Mandys, V., Gispen, W.H., et al. (2006) Cisplatin-Induced Apoptosis in Cultures of Human Schwann Cells. Neuroscience Letters, 392, 22-26.
https://doi.org/10.1016/j.neulet.2005.08.068
[12]
Kartalou, M. and Essigmann, J.M. (2001) Mechanisms of Resistance to Cisplatin. Mutation Research, 478, 23-43. https://doi.org/10.1016/S0027-5107(01)00141-5
[13]
McGuire, S. (2016) World Cancer Report 2014. Geneva, Switzerland: World Health Organization, International Agency for Research on Cancer, WHO Press, 2015. Advances in Nutrition, 7, 418-419. https://doi.org/10.3945/an.116.012211
[14]
Menezes, C.S.R., de Paula Costa, L.C.G., de Melo Rodrigues ávila, V., et al. (2007) Analysis in Vivo of Antitumor Activity, Cytotoxicity and Interaction between Plasmid DNA and the cis-Dichloro-Tetra-Ammine-Ruthenium(III) Chloride. Chemico-Biological Interactions, 167, 116-124. https://doi.org/10.1016/j.cbi.2007.02.003
[15]
Novakova, O., Kasparkova, J., Vrana, O., et al. (1995) Correlation between Cytotoxicity and DNA Binding of Polypyridyl Ruthenium Complexes. Biochemistry, 34, 12369-12378. https://doi.org/10.1021/bi00038a034
[16]
Pereira, F.C., de Lima, A.P., Vilanova-Costa, C.A.S.T., et al. (2014) Cytotoxic Effects of the Compound cis-Tetraammine(Oxalato)ruthenium(III) Dithionate on K-562 Human Chronic Myelogenous Leukemia Cells. SpringerPlus, 3, Article No. 301.
https://doi.org/10.1186/2193-1801-3-301
[17]
Rosenberg, B., Vancamp, L., Trosko, J.E., et al. (1969) Platinum Compounds: A New Class of Potent Antitumour Agents. Nature, 222, 385-386.
https://doi.org/10.1038/222385a0
[18]
Bogado, A.L., de Souza, R.F., Schuchardt, U. and Batista, A.A. (2003) On the Kinetics of Epoxidation of Olefins by cis and trans-[RuCl2(dppb)(2, 2’-bipy)] Complexes. Journal of Molecular Catalysis A: Chemical, 203, 129-135.
https://doi.org/10.1016/S1381-1169(03)00353-4
[19]
Tarso, M.F., Dourado, G.A., Batista, F.D., et al. (2020) Synthesis, Characterization, and Evaluation of Antitumor Potential in MCF-7 Cells of Ruthenium-Derived Compounds. Advances in Biological Chemistry, 10, 86-98.
https://doi.org/10.4236/abc.2020.103007
[20]
Torre, L.A., Bray, F., Siegel, R.L., et al. (2015) Global Cancer Statistics, 2012. CA, 65, 87-108. https://doi.org/10.3322/caac.21262
[21]
van Vliet, P.M., Toekimin, S.M.S., Haasnoot, J.G., et al. (1995) mer-[Ru(terpy)Cl3] (terpy=2,2’:6’,2”-terpyridine) Shows Biological Activity, Forms Interstrand Cross-Links in DNA and Binds Two Guanine Derivatives in a Trans Configuration. Inorganica Chimica Acta, 231, 57-64. https://doi.org/10.1016/0020-1693(94)04320-U
[22]
Vilanova-Costa, C.A.S.T., Porto, H.K.P., de Castro Pereira, F., et al. (2014) The Ruthenium Complexes cis-(Dichloro)Tetramineruthenium(III) Chloride and cis-Tetraammine(Oxalato)Ruthenium(III) Dithionate Overcome Resistance Inducing Apoptosis on Human Lung Carcinoma Cells (A549). BioMetals, 27, 459-469.
https://doi.org/10.1007/s10534-014-9715-x
[23]
Vilanova-Costa, C.A.S.T., Porto, H.K.P., Pereira, L.C.G., et al. (2015) MDR1 and Cytochrome P450 Gene-Expression Profiles as Markers of Chemosensitivity in Human Chronic Myelogenous Leukemia Cells Treated with Cisplatin and Ru(III) Metallocomplexes. Biological Trace Element Research, 163, 39-47.
https://doi.org/10.1007/s12011-014-0133-2
