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LncRNA ATB、LncRNA GAS5、LncRNA P21、LncRNA MEG3在绝经期子宫肌瘤患者中的表达及临床意义研究
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Abstract:
目的：探究LncRNA ATB、LncRNA GAS5、LncRNA P21、LncRNA MEG3在绝经期子宫肌瘤(Uterus fibroid, UFs)患者的表达及临床意义，以期为临床上UFs的诊断和治疗提供新的思路和方法。方法：选择自2018年1月至2020年1月收入我院妇科的34例符合条件的子宫肌瘤患者为研究对象，并且选取同期54例因子宫脱垂而行子宫切除术的患者作为对照组，收集入组者的子宫组织和血清样本。利用RT-PCR检测样本中LncRNA ATB、LncRNA GAS5、LncRNA P21、LncRNA MEG3的差异性表达，Logistic回归分析与子宫肌瘤相关的危险因素。结果：两组患者的BMI，妊娠史以及血清学指标雌激素(E2)，促黄体生成素(LH)，促卵泡激素(FSH)和孕酮(P)比较具有统计学差异(P < 0.05)。宫肌瘤组患者血清和子宫组织中LncRNA ATB、LncRNA GAS5、LncRNA P21、LncRNA MEG3的mRNA相对表达量要明显低于正常对照组，比较具有统计学差异(P < 0.05)。Logistic分析结果显示，高BMI，有妊娠史，E2高水平，P高水平和LncRNA ATB、LncRNA GAS5、LncRNA P21、LncRNA MEG3低表达是子宫肌瘤发生的高危因素(P < 0.05)。结论：雌激素(E2)和孕酮(P)含量的升高，LncRNA ATB、LncRNA GAS5、LncRNA P21、LncRNA MEG3的降低与子宫肌瘤的发生密切相关，是临床上子宫肌瘤早期诊断和早期治疗新的靶点。
Objective: To explore the expression and clinical significance of LncRNA ATB, LncRNA GAS5, LncRNA P21 and LncRNA MEG3 in uterine leiomyoma (UFs), so as to provide new ideas and methods for clinical diagnosis and treatment of UFs. Methods: From January 2018 to January 2020, 34 patients with uterine fibroids in our hospital were selected as the research objects, and 54 patients who underwent hysterectomy due to uterine prolapse in the same period were selected as the control group. The uterine tissue and serum samples of the patients were collected. The RT-PCR was used to detect the differential expression of LncRNA ATB, LncRNA GAS5, LncRNA P21 and LncRNA MEG3 in the samples, and the basic information of patients was collected. Logistic regression analysis of risk factors associated with hysteromyoma. Results: There were significant differences in BMI, pregnancy history, serum estrogen (E2), luteinizing hormone (LH), follicle stimulating hormone (FSH) and progesterone (P) between the two groups (P < 0.05). The relative mRNA expression levels of LncRNA ATB, LncRNA GAS5, LncRNA P21 and LncRNA MEG3 in serum and uterine tissue of hysteromyoma group were significantly lower than those of normal control group (P < 0.05). The Logistic analysis showed that high BMI, history of pregnancy, high level of E2, high level of P and low expression of LncRNA ATB, LncRNA GAS5, LncRNA P21 and LncRNA MEG3 were the risk factors of uterine fibroids (P < 0.05). Conclusion: The increase of estrogen (E2) and progesterone (P), and the decrease of LncRNA ATB, LncRNA GAS5, LncRNA P21 and LncRNA MEG3 are closely related to the occurrence of hysteromyoma. They are new targets for early diagnosis and treatment of hysteromyoma.
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