Phytochemical Analysis and Bioactivity Screening of Primary and Secondary Metabolic Products of Medicinal Plants in the Valleys of Medina Region Saudi Arabia
Medicinal plants are highly
valued for their active compounds. These plants can be used in various fields and preservation of these plants in
their environment. The present study aimed to screen medicinal plants used in
traditional medicine in Medina valleys for the presence of metabolites, and to
answer the following question: is the ethnomedicinal importance of medicinal
plants used in Medina valleys conform to their primary and secondary metabolite content. Eight plants (Pulicariaincise, Heliotropiumarbainense, Commicarpusgrandiflorus, Rumexvesicarius, Sennaalexandrina, Rhazyastricta, Withaniasomniferaand Asphodelusfistulosus) were collected fromthe Medina valleys and were biochemically analyzed to determine the
different compounds after leaves extraction analyzed statistically to clarify
the content of primary compounds. The
chemical compounds in the most active fraction were determined using
quantitative phytochemical and gas chromatography-mass spectrometry
(GC/MS) analytical methods, comparing the mass spectra of the GC/MS identified
compounds with those of the Center of Excellence in Environmental Studies
(CEES) database library. The result showed 16 aroma compounds representing the
GC/MS analysis revealed the presence of various compounds like 4,4-Dimethyl
octane, 5H-1-Pyrindine and 1,3-Cyclopentadiene,
1,2,5,5-tetramethyl- in the ethanolic extract of Pulicaria incisa. The
most prevalent plants were Pulicaria incisa, Sennaalexandrina and Heliotropiumarbainense the study plants have high content of protein. There is a need to focus phytochemical screening
References
[1]
Hussein, R.A. and El-Anssary, A.A. (2019) Plants Secondary Metabolites: The Key Drivers of the Pharmacological Actions of Medicinal Plants. In: Builders, P.F., Ed., Herbal Medicine, IntechOpen, London, 11-30. https://doi.org/10.5772/intechopen.76139
[2]
Vieira, A.A. (2010) Comparison of Traditional Anti-Inflammation and Anti-Infection Medicinal Plants with Current Evidence from Biomedical Research: Results from a Regional Study. Pharmacognosy Research, 2, 293-295. https://doi.org/10.4103/0976-4836.72326
[3]
Hoekou, Y.P., Tchacondo, T., Karou, S.D., Koudouvo, K., Atakpama, W., Pissang, P., Gbogbo, A.K., Woegan, A.Y., Batawila, K. and Akpagana, K. (2016) Ethnobotanical Study of Latex Plants in the Maritime Region of Togo. Pharmacognosy Research, 8, 128-134. https://doi.org/10.4103/0974-8490.175613
[4]
Khan, S., Alghafari, Y. and Kha, S. (2016) Temperature and Precipitation Fluctuation of Madinah-Al-Munawara, Kingdom of Saudi Arabia (1959-2011). Atmospheric and Climate Sciences, 6, 402-414. https://doi.org/10.4236/acs.2016.63033
[5]
Collenette, S. (1985) An Illustrated Guide to the Flower of Saudi Arabia Meteorology and Environmental Protection Administration, Kingdom of Saudi Arabia. Floral Publication No. 1985. Scorpion Publishing, London.
[6]
Jamshidi-Kia, F., Lorigooini, Z. and Amini-Khoei, H. (2018) Medicinal Plants: Past History and Future Perspective. Journal of HerbMed Pharmacology, 7, 1-7. https://doi.org/10.15171/jhp.2018.01
[7]
Mbadianya, J., Echezona, B., Ugwuoke, K. and Wokocha, R. (2013) Phytochemical Constituents of Some Medicinal Plants. International Journal of Science and Research (IJSR), 2, 18-22. https://doi.org/10.15373/22778179/JUNE2013/1
[8]
Rahman, M.A., Mossa, J.S., Al-Said, M.S. and Al-Yahya, M.A. (2004) Medicinal Plant Diversity in the Flora of Saudi Arabia 1: A Report on Seven Plant Families. Fitoterapia, 75, 149-161. https://doi.org/10.1016/j.fitote.2003.12.012
[9]
Yusuf, M., Al-Oqail, M.M., Al-Sheddr, E.S., Al-Rehaily, A.J. and Rahman, M.A. (2014) Diversity of Medicinal Plants in the Flora of Saudi Arabia 3: An Inventory of 15 Plant Families and Their Conservation Management. International Journal of Environment, 3, 312-320. https://doi.org/10.3126/ije.v3i3.11091
[10]
Awadh, A.N., Mansi, N., Ahmed, S., Alghamdi, R., Abu, A., Al-Khulaidi, A. and Sirajudheen, A. (2019) Ultra-High Performance Liquid Chromatography-Electrospray Ionization-Mass Spectroscopy Quantification, Xanthine Oxidase Inhibitory, and Antioxidant Activity Profile of Some Medicinal Plants from Al-Baha Region. Pharmacognosy Research, 15, 162-167.
[11]
Yassin, M.A., Salih, A.B. and Hosny, A.M. (2013) Academic Medicinal Plants in Saudi Arabia: I. Sarrwat Mountains at Taif, KSA. Journal of Plant Sciences, 6, 134-145.
[12]
Afef, L., Faten, O., Marina, D., Armando, Z., Simona, Z. and Rabiaa, H. (2013) Phytotoxic Activity of Cleome arabica L. and Its Principal Discovered Active Compounds. South African Journal of Botany, 88, 341-351. https://doi.org/10.1016/j.sajb.2013.08.016
[13]
Migahid, A.M. (1996) Flora of Saudi Arabia. 4th Edition, Vol. 1, 2, 3, King Saud University, Riyadh.
[14]
Collenette, S. (1999) Wildflowers of Saudi Arabia. National Commission for Wildlife Conservation and Development (NCWCD), Riyadh.
[15]
Chaudhary, S.A. (2001) Flora of the Kingdom of Saudi Arabia. Vol. 1, 2, 3, National Agriculture and Water Research Center, National Herbarium, Ministry of Agriculture and Water, Riyadh.
[16]
Migahid, M.M. and Aljeddani, G.S. (2019) Ecological Response to Habitat Variation for Perennial Species in Saudi Arabia. Life Science Journal, 16, 69-78.
[17]
Khayyat, S., Al-Kattan, M. and Basudan, N. (2018) Phytochemical Screening and Antidermatophytic Activity of Lavender Essential oil from Saudi Arabia. International Journal of Pharmacology, 14, 802-810. https://doi.org/10.3923/ijp.2018.802.810
[18]
Bradford, M.M. (1976) A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle Protein-Dye Binding. Analytical Biochemistry, 12, 248-254. https://doi.org/10.1016/0003-2697(76)90527-3
[19]
Bates, L.S., Waldern, R.P. and Teare, I.D. (1973) Rapid Determination of Free Proline for Water Stress Studies. Plant and Soil, 39, 205-207. https://doi.org/10.1007/BF00018060
[20]
Ya, P.L. and Tunekazu, T. (1966) An Improved Colorimetric Determination of Amino Acids with the Use of Ninhydrin. Analytical Biochemistry, 14, 71-77. https://doi.org/10.1016/0003-2697(66)90057-1
[21]
Muilling, S.R. and Parish, J.H. (1984) New Reducing Sugar Assay for the Study of Cellulases. Enzyme and Microbial Technology, 6, 491-496. https://doi.org/10.1016/0141-0229(84)90002-4
[22]
Gul, R., Jan, S.U., Syed, F., Sherani, F. and Nusrat, J. (2017) Preliminary Phytochemical Screening, Quantitative Analysis of Alkaloids, and Antioxidant Activity of Crude Plant Extracts from Ephedra intermedia Indigenous to Balochistan. The Scientific World Journal, 2017, Article ID: 5873648. https://doi.org/10.1155/2017/5873648
[23]
Singh, V. and Kumar, R. (2017) Study of Phytochemical Analysis and Antioxidant Activity of Allium sativum of Bundelkhand Region. International Journal of Life Sciences Scientific Research, 3, 1451-1458. https://doi.org/10.21276/ijlssr.2017.3.6.4
[24]
Harborne, J.B. (1998) Phytochemical Methods. Chapman and Hall Ltd., London, 100-200.
[25]
Tiwari, P., Kumar, B., Kaur, M., Kaur, G. and Kaur, H. (2011) Phytochemical Screening and Extraction: A Review. Internationale Pharmaceutica Sciencia, 1, 98-106.
[26]
Audu, S.A., Mohammad, I. and Kaita, H.A. (2007) Phytochemical Screening of the Leaves of Lophira lanceolata (Ochanaceae). Life Science Journal, 4, 75-79.
[27]
Kumar, R., Sharma, S. and Devi, L. (2018) Investigation of Total Phenolic, Flavonoid Contents and Antioxidant Activity from Extract of Azadirachta indica of Bundelkhand Region. International Journal of Life Sciences and Scientific Resesrch, 4, 1925-1933. https://doi.org/10.21276/ijlssr.2018.4.4.10
[28]
Uma, K.S., Parthiban, P. and Kalpana, S. (2017) Pharmacognostical and Preliminary Phytochemical Screening of Aavaarai Vidhai Chooranam. Asian Journal of Pharmaceutical and Clinical Research, 10, 111-116. https://doi.org/10.22159/ajpcr.2017.v10i10.19422
[29]
Hanane, L., Redouane, B., Rajaa, K., Ebich, F., El Hassan, A. and Mohamed, F. (2022) Chemical Composition and Insecticidal Potential of Pulicaria incisa (Lam) Essential Oil from Moroccan Plant Against Sitophilus oryzae (L.) and Tribolium castaneum (Herbst.). Biointerface Research in Applied Chemistry, 12, 2262-2274. https://doi.org/10.33263/BRIAC122.22622274
[30]
Wani, S., Ahmad, P., Zargar, S. and Dar, T. (2019) Therapeutic Potential of Medicinal Plant Proteins: Present Status and Future Perspectives. Current Protein & Peptide Science, 20, 443-487.
[31]
Szabados, L. and Savoure, A. (2010) Proline: A Multifunctional Amino Acid. Trends in Plant Science, 15, 89-97. https://doi.org/10.1016/j.tplants.2009.11.009
[32]
Moran, E., Tortoledo, O., Yañez, G., Zamora-Alvarez, L., Stephens-Camacho, N., Soñanez-Organis, J., Ochoa-Lopez, L. and Rosas-Rodríguez, J. (2014) Determination of Amino Acids in Medicinal Plants from Southern Sonora, Mexico. Tropical Journal of Pharmaceutical Research, 13, 601-606. https://doi.org/10.4314/tjpr.v13i4.17
[33]
Trouvelot, S., Héloir, M.C., Poinssot, B., Gauthier, A., Paris, F., Guillier, C., Combier, M., Trdá, L., Daire, X. and Adrian, M. (2014) Carbohydrates in Plant Immunity and Plant Protection: Roles and Potential Application as Foliar Sprays. Frontiers in Plant Science, 5, Article No. 592. https://doi.org/10.3389/fpls.2014.00592
[34]
Makhmudova, U., Schulze, P., Lütjohann, D. and Weingürtner, O. (2021) Phytosterols and Cardiovascular Disease. Current Atherosclerosis Reports, 23, Article No. 68. https://doi.org/10.1007/s11883-021-00964-x
[35]
Kritchevsky, D. and Chen, S. (2005) Phytosterols-Health Benefits and Potential Concerns: A Review. Nutrition Research, 25, 413-428. https://doi.org/10.1016/j.nutres.2005.02.003
[36]
Rascón, V., Luisa, T.M., Heriberto, V.C., Garibay, E., Adriana, R. and Zepeda, R. (2016) Triterpenoids: Synthesis, Uses in Cancer Treatment and Other Biological Activities. In: Berhardt, L.V., Ed., Advances in Medicine and Biology, Nova Science Publishers, Hauppauge, 139-181.
[37]
Pathak, D., Pathak, K. and Singla, A.K. (1991) Flavonoids as Medicinal Agents-Recent Advances. Fitoterapia, 62, 371-389.
[38]
Arts, I.C. and Hollman, P.C. (2005) Polyphenols and Disease Risk in Epidemiologic Studies. The American Journal of Clinical Nutrition, 81, S317-S325. https://doi.org/10.1093/ajcn/81.1.317S
[39]
Schneider, G. and Wolfling, J. (2004) Synthetic Cardenolides and Related Compounds. Current Organic Chemistry, 8, 1381-1403. https://doi.org/10.2174/1385272043369926
[40]
Ayoola, G.A., Coker, H.A.B., Adesegun, S.A., Adepoju-Bello, A.A., Obaweya, K., Ezennia, E.C. and Atangbayila, T.O. (2008) Phytochemical Screening and Antioxidant Activities of Some Selected Medicinal Plants Used for Malaria Therapy in Southwestern Nigeria. Tropical Journal of Pharmaceutical Research, 7, 1019-1024. https://doi.org/10.4314/tjpr.v7i3.14686
[41]
El Aziz, M., Ashour, A.S. and Melad, A.S.G. (2019) A Review on Saponins from Medicinal Plants: Chemistry, Isolation, and Determination. Journal of Nanomedicine Research, 8, 282-288. https://doi.org/10.15406/jnmr.2019.07.00199
[42]
Panche, A.N., Diwan, A.D. and Chandra, S.R. (2016) Flavonoids: An Overview. Journal of Nutritional Science, 5, e47. https://doi.org/10.1017/jns.2016.41
[43]
Mohan, E., Suriya, S., Shanmugam, S. and Rajendran, K. (2021) Qualitative Phytochemical Screening of Selected Medicinal Plants. Journal of Drug Delivery and Therapeutics, 11, 141-144. https://doi.org/10.22270/jddt.v11i2.4609
[44]
Ramírez, P., Cesar Martínez, Q., Janio, G. and Jesús, D. (2019) Coumarins from the Peel of Citrus Grown in Colombia: Composition, Elicitation and Antifungal Activity. Heliyon, 5, e01937. https://doi.org/10.1016/j.heliyon.2019.e01937
[45]
Li, H., Wang, Z. and Liu, Y. (2003) Review in the Studies of Tannins Activity of Cancer Prevention and Anti-Cancer. Journal of Chinese Medicinal Materials, 26, 444-448.
[46]
Sharma, K., Kumar, V., Kaur, J., Tanwar, B., Goyal, A., Sharma, R., Gat, Y. and Kumar, A. (2019) Health Effects, Sources, Utilization and Safety of Tannins: A Critical Review. Toxin Reviews, 91, 1-13. https://doi.org/10.1080/15569543.2019.1662813
[47]
Muhammad, K.G., Muhammad, A.G., Sajid, N.H., Majid, M., Mukhayar, A. and Wajid, S. (2016) Ethnopharmacological, Phytochemical and Pharmacognostic Potential of Genus Heliotropium L. Turkish Journal of Pharmaceutical Sciences, 13, 259-280. https://doi.org/10.5505/tjps.2016.20591
[48]
Al-Duais, M., Müler, L., Böhm, V. and Jetschke, G. (2009) Antioxidant Capacity and Total Phenolics of Cyphostemma digitatum before and after Processing—Use of Different Assays. European Food Research and Technology, 228, 813-821. https://doi.org/10.1007/s00217-008-0994-8
[49]
Khare, C.P. (2007) Indian Medicinal Plants: An Illustrated Dictionary. Springer, New York. https://doi.org/10.1007/978-0-387-70638-2
[50]
Gerard, H. and Tillett, S. (2010) El Jardín Medicinal de la Revista El Palo de arco, el Sen, el Yagrumo y la Zarzaparrilla. Revista Facultad de Farmacia, 73, 43-49.
[51]
Iqbal, S., Bhanger, M.I., Akhtar, M., Anwa, F., Ahmed, K.R. and Anwer, T. (2006) Antioxidant Properties of Methanolic Extracts from Leaves of Rhazya stricta. Journal of Medicinal Food, 9, 270-275. https://doi.org/10.1089/jmf.2006.9.270
[52]
Bukhari, N.A., Al-Otaibi, R.A. and Ibhrahim, M.M. (2017) Phytochemical and Taxonomic Evaluation of Rhazya stricta in Saudi Arabia. Saudi Journal of Biological Sciences, 24, 1513-1521. https://doi.org/10.1016/j.sjbs.2015.10.017
[53]
Said, K.I. (2013) Quantitive Evolution of with Anolides Content of Egyptian Withania somnifera (L.) Roots. Al-Azhar Journal of Pharmaceutical Sciences, 47, 95-104. https://doi.org/10.21608/ajps.2013.7112
[54]
Ayoub, K., Emira, N., Soumia, B., Kaïss, A., Bouslama, L., Mohd, A. andrea, D., Adel, K., Mejdi, S., Mushtaq, A.K. and Ines, M. (2021) LC-ESI/MS-Phytochemical Profiling with Antioxidant, Antibacterial, Antifungal, Antiviral and in Silico Pharmacological Properties of Algerian Asphodelus tenuifolius (Cav.) Organic Extracts. Antioxidants, 10, Article No. 628. https://doi.org/10.3390/antiox10040628
