Background: MicroRNAs (miRs) are noncoding gene regulators that
may have a role as diagnostic or prognostic biomarkers in systemic lupus
erythematosus (SLE). Aim: To measure the blood levels of miR-146a,
miR-126 and miR-30a in Sudanese SLE patients and to investigate their potential
role in disease pathogenesis and utility as biomarkers for SLE. Material and
Methods: A total of48 SLE
patients and 20 matched healthy individuals were enrolled in this study. SLE
disease activity index (SLEDAI) was assessed. The blood levels of miR-146a,
miR-126 and miR-30a were determined by Real-time polymerase chain
reaction (PCR) in all participants. Γ-INF and IL-2 were analyzed by ELISA, and
CD markers were used in flowcytometry. Results: The mean age of the patients was 31.5 ± 8.5 years with disease
duration > 5 years. In SLE patients, the mean blood level fold changes of
miR-146a (0.33 ± 0.277; P < 0.001), miR-126 (2.44 ± 1.771; P = 0.007) and
miR-30a (1.56 ± 1.40; P > 0.305) compared to controls. Down regulation of
miR-146a increase expression of γ-INF
(P < 0.002), whereas the up regulation of miR-126 increase expression of CD
markers (P < 0.000) in SLE patients.MiR-126 at a cut-off value 1.209 and miR-146a at
cut-off value of 0.9233 which can discriminate between SLE patients
significantly associated with SLE disease. Conversely, miR-30a was
insignificantly associated with SLE disease (P value > 0.05) as no
differences between the SLE patients and healthy control. Conclusion:
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