Background: Von Willebrand Factor (VWF) is a large multimeric
plasmagly-coprotein that plays an important role in primary haemostasis. VWF
together with ADAMTS13 plays a key role in arterial thrombosis. VWF is integral
for platelet adhesion to collagen fibres and atherosclerotic plaues and platelets
aggregation under high shear conditions. Upon vascular wall damage, plasma VWF
binds to collagen in the exposed endothelial matrix and platelet glycoprotein
triggers platelet aggregation and thrombous formation. In the early stages of acute
ischaemic stroke, VWF increased significantly and the ADAMTS13 level decreased.
The increase of plasma VWF and FVIII in a patient with ischaemic stroke indicated an increased
risk of complications and severe neurological deficit. Objective: to evaluate the validity of VWF and ADFAMTS13 as
diagnostic markers of cerebral infarction by comparing the two markers’ validity in diagnosis. Methods: this case
control study enrolled 38 cerebral infarction patients 20 acute infarction and 18
recurrent infarction. Patients were compared with 65 age and sex with control
patients. We performed the VWF assay using the minividas immunofluorescence technique and the ADAMTS13 assay using the ELISA technique.Results: VWF level of acute infarction patients (280.97 ± 109.83 IU/dl) while its
level in recurrent
References
[1]
Nieswandt, B., Pleine, I. and Bonder, M. (2011) Platelet Adhesion and Activation Mechanism in Arterial Thrombosis and Ischaemic Stroke. Journal of Thrombosis and Haemostasis, 9, 92-104. https://doi.org/10.1111/j.1538-7836.2011.04361.x
[2]
Schuppner, R., Dirks, M. and Grosse, S.M. (2018) ADAMTS-13 Activity Predicts Outcome in Acute Ischaemic Stroke Patients Undergoing Endovascular Treatment. Thrombosis and Haemostasis, 118, 758-767. https://doi.org/10.1055/s-0038-1637732
[3]
Favresse, J., Lardinois, B., Chatelain, B., Jacqmin, H. and Mullier, F. (2018) Evaluation of the Fully Automated HemosIL Acustar ADAMTS13 Activity Assay. Thrombosis and Haemostasis, 118, 942-944. https://doi.org/10.1055/s-0038-1641151
[4]
Donkel, S.J., Benaddi, B. and Dippel, D.W. (2019) Prognostic Haemostasis Biomarkers in Acute Ischaemic Stroke: A Systematic Review. Arteriosclerosis, Thrombosis, and Vascular Biology, 39, 360-372. https://doi.org/10.1161/ATVBAHA.118.312102
[5]
Alonso, A., Jang, W., Agarwal, S.K. and Soliman, E.Z. (2012) Hemostatic Markers Associated with the Risk and Prognosis of Arterial Fibrillation: The ARIC Study. International Journal of Cardiology, 155, 217-222. https://doi.org/10.1016/j.ijcard.2010.09.051
[6]
Xu, H., Cao, Y. and Yang, X. (2017) ADAMTS13 Controls Vascular Remodeling by Modifying VWF Reactivity during Stroke Recovery. Blood, 130, 11-22. https://doi.org/10.1182/blood-2016-10-747089
[7]
Maino, A., Rosendaal, F.R., Algra, A., Peyvandi, F. and Siegerink, B. (2015) Hypercoagulability Is a Stronger Risk Factor for Ischaemic Stroke than for Myocardial Infarction: A Systematic Review. PLOS ONE, 10, e0133523. https://doi.org/10.1371/journal.pone.0133523
[8]
Sonneveld, M.A., De Maat, M.P., Portegies, M.L., Kavousi, M. and Hofman, A. (2015) Low ADAMTS13 Activity Is Associated with an Increased Risk of Ischemic Stroke. Blood, 126, 2739-2746. https://doi.org/10.1182/blood-2015-05-643338
[9]
Samai, A., Monlezun, D., Shaban, A., George, A. and Dowell, L. (2014) Von Willebrand Factor Drives the Association between Elevated Factor VIII and Poor Outcomes in Patients with Ischemic Stroke. Stroke, 45, 2789-2791. https://doi.org/10.1161/STROKEAHA.114.006394
[10]
Cai, P., Luo, H., Xu, H., Zhu, X. and Xu, W. (2015) Recombinant ADAMTS 13 Attenuates Brain Injury after Intracerebral Hemorrhage. Stroke, 46, 2647-2653. https://doi.org/10.1161/STROKEAHA.115.009526
[11]
Liu, W. and Benshu, Z. (2019) VWF/ADAMTS13 and Ischemic Stroke. Biomedical Jouranl of Scientific & Technical Research, 18, 13480-13482. https://doi.org/10.26717/BJSTR.2019.18.003134
[12]
Undas, A. (2017) Prothrombotic Fibrin Clot Phenotype in Patients with Deep Vein Thrombosis and Pulmonary Embolism: A New Risk Factor for Recurrence. Biomed Research International, 2017, Article ID: 8196256. https://doi.org/10.1155/2017/8196256
[13]
Undas, A., Podolec, P., Zawilska, K., Pieculewicz, M., Jedlinski, I. and Stepien, E. (2009) Altered Fibrin Clot Structure/Function in Patients with Cryptogenic Ischemic Stroke. Stroke, 40, 1499-1501. https://doi.org/10.1161/STROKEAHA.108.532812
[14]
Roy-O’Reilly, M. and McCullough, L.D. (2018) Age and Sex Are Critical Factors in Ischemic Stroke Pathology. Endocrinology, 159, 3120-3131. https://doi.org/10.1210/en.2018-00465
[15]
Kathryn, M., Rexrode, T.E. and Madsen, A.G. (2022) The Impact of Sex and Gender on Stroke. Circulation Research, 130, 512-528. https://doi.org/10.1161/CIRCRESAHA.121.319915
[16]
Gaignard, P., Lere, P. and Therond, P. (2017) Role of Sex Hormones on Brain Mitochondrial Function with Special Reference to Aging and Neurodegeneration Diseases. Frontiers in Aging Neuroscience, 9, Article 406. https://doi.org/10.3389/fnagi.2017.00406
[17]
Amiri-Nikpour, M.R., Mazarbaghi, S. and Hamdi-Hollssou, M. (2015) An Open-Label Evaluator-Blinded Clinical Study of Minocycline Neuroprotection in Ischaemic Stroke, Gender-Dependent Effect. Acta Neurologica Scandinavica, 131, 45-50. https://doi.org/10.1111/ane.12296
[18]
Mazune, C.M. and Jones, D.P. (2015) Twenty Years and Still Counting: Including Women as Participants and Studying Sex and Gender in Biomedical Research. BMC Women’s Health, 15, Article No. 94. https://doi.org/10.1186/s12905-015-0251-9
[19]
Edward, C., Jauch, M.G. and Faha, F.A. (2022) What Is the Role of Lab Testing in the Working of Ischaemic Stroke. https://www.medscape.com/answers/1916852-118655
[20]
Xu, H., Xingquan, Z., David, W. and Churnyue, W. (2013) Association of Hypertension with Stroke Recurrence Depends on Ischaemic Stroke Subtype. Stroke, 44, 1232-1237. https://doi.org/10.1161/STROKEAHA.111.000302
[21]
Martínez-Martínez, M., Cazorla-García, R., Rodríguez de Antonio, L.A., Martínez-Sánchez, P., Fuentes, B. and Diez-Tejedor, E. (2010) Hypercoagulability and Ischemic Stroke in Young Patients. Neurología, 25, 343-348. https://doi.org/10.1016/S2173-5808(10)70065-1
[22]
Tanaka, S., Takashi, H., Manaba, H. and Katsumi, W. (2009) Enhancement of Pinch Force in the Lower Leg by a Nodal Transcranial Direct Current. Experimental Brain Research, 196, 459-465. https://doi.org/10.1007/s00221-009-1863-9
[23]
Antonio, M., Andrea, C. and Elisa, F. (2014) Changes of Liver Enzymes and Bilirubin during Ischemic Stroke: Mechanisms and Possible Significance. BMC Neurology, 14, Article No. 122. https://doi.org/10.1186/1471-2377-14-122
[24]
Robba, C., Batlglin, D., Pelos, P., Samary, C.S., Silva, P.L., Ball, L., et al. (2020) Ischaemic Stroke Induced Distal Damage, Pathophysology and New Therapeutic Strategies. Intensive Care Medicine Experimental, 8, Article No. 23. https://doi.org/10.1186/s40635-020-00305-3
[25]
Ormstad, H., Aass, H.C. and Lund-Sonenien, N. (2011) Serum Level of Cytokines and C-Reactive Protein in Acute Ischaemic Stroke Patients and Their Relationship to Stroke Lateralization Type and Infarct Volume. Journal of Neurology, 258, 677-685. https://doi.org/10.1007/s00415-011-6006-0
[26]
Zhao, B.Q., Chauhan, A.K., Canault, M., Patten, I.S., Yang, J.J., Dockal, M., Scheiflinger, F. and Wagner, D.D. (2009) Von Willebrand Factor-Cleaving Protease ADAMTS13 Reduces Ischemic Brain Injury in Experimental Stroke. Blood, 114, 3329-3334. https://doi.org/10.1182/blood-2009-03-213264
[27]
Chen, X., Cheng, X., Zhang, S.F. and Danhon, W. (2019) ADAMTS13: An Emerging Target in Stroke Therapy. Frontiers in Neurology, 10, Article 772. https://doi.org/10.3389/fneur.2019.00772
[28]
Gogia, S., Kelkan, A. and Zhang, C. (2017) Role of Calcium in Regulating the Intra and Extracellular Cleavage of Von-Willbrand Factor by the Protease ADAMTS13. Blood Advances, 1, 2063-2074. https://doi.org/10.1182/bloodadvances.2017009027
[29]
Michelle, A.M., Sonaeveld Frank, W.G. and Moned, P.M. (2015) Low ADAMTS13 Activity Is Associated with an Increased Risk of Ischaemic Stroke. Blood, 126, 2739-2746. https://doi.org/10.1182/blood-2015-05-643338
[30]
Manasa, K.M., Gagan, D.F. and Anil, L.C. (2022) Constitutively Active ADAMTS13: An Emerging Thrombolytic Agent for Acute Ischaemic Stroke. Journal of Thrombosis and Haemostasis, 20, 790-793. https://doi.org/10.1111/jth.15649
