Background:Escherichiacoli are ubiquitous
bacteria colonising both humans and animals. Extended spectrum β-lactamase-producing E.coli has been selected as a
suitable indicator for the monitoring and surveillance of antimicrobial
resistance. Death due to resistant bacteria is continuously rising in Cameroon, but the contribution of the aviary sector is not well studied.
Therefore, this study aimed to investigate the resistance profile of extended
spectrum beta-lactamases-producing Escherichiacoli strains, isolated from faeces of broiler chickens in
Yaoundé, capital city of Cameroon. Methods: A cross-sectional
descriptive study was carried out from February to June 2020. Escherichiacoli were isolated from
samples of broilers in poultry farms in Yaoundé and submitted to the extended
spectrum β-lactamase screening. The
logistic regression was used to assess the statistical association of a
significance threshold p-value of 0.05. Results: Out of 385 faecal
samples collected in broiler farms, 114 Escherichiacoli isolates were obtained
out of which 30 (26.32%) were Extended Spectrum Beta-Lactamases-producing Escherichiacoli. These isolates revealed
high resistance to all antibiotic families. Poor storage conditions for feeds
and the proximity to latrines, the troughs on the ground, the lack of foot bath
and uniforms, the inadequate treatment of faeces, the poor usage of preventive
antibiotics and the lack of water treatment have been identified as risk
factors to faecal carriage of ESBL-producing Escherichiacoli. Conclusion: This
work reveals the emergence of Extended Spectrum Beta-Lactamases-producing Escherichiacoli in poultry farms in
Yaoundé and the failure in the biosecurity system. As such, the awareness of
poultry breeders on the respect of biosecurity measures may be an effective
tool to tackle antimicrobial resistance, specifically in livestock industries
using a One Health approach.
References
[1]
Food and Agriculture Organization (2020) Lives Animals and Livestock Primary in Cameroon: Producion Animal/Slaughterered and Stocks. FAO, Rome.
[2]
Furtula, V., Farrell, E.G., Diarrassouba, F., Rempel, H., Pritchard, J. and Diarra, M.S. (2010) Veterinary Pharmaceuticals and Antibiotic Resistance of Escherichia coli Isolates in Poultry Litter from Commercial Farms and Controlled Feeding Trials. Poultry Science, 89, 180-188. https://doi.org/10.3382/ps.2009-00198
[3]
Kumar, K., Gupta, S.C., Chander, Y. and Singh, A.K. (2005) Antibiotic Use in Agriculture and Its Impact on the Terrestrial Environment. Advances in Agronomy, 87, 1-54. https://doi.org/10.1016/S0065-2113(05)87001-4
[4]
Diarra, M.S., Silversides, F.G., Diarrassouba, F., Pritchard, J., Masson, L., Brousseau, R., Bonnet, C., Delaquis, P., Bach, S., Skura, B.J. and Topp, E. (2007) Impact of Feed Supplementation with Antimicrobial Agents on Growth Performance of Broiler Chickens, Clostridium perfringens and Enterococcus Counts, and Antibiotic Resistance Phenotypes and Distribution of Antimicrobial Resistance Determinants in Escherichia coli Isolates. Applied and Environmental Microbiology, 73, 6566-6576. https://doi.org/10.1128/AEM.01086-07
[5]
Vounba, P., Rhouma, M., Arsenault, J., Alambédji, R.B., Fravalo, P. and Fairbrother, J.M. (2019) Prevalence of Colistin Resistance and mcr-1/mcr-2 Genes in Extended- Spectrum β-Lactamase/AmpC-Producing Escherichia coli Isolated from Chickens in Canada, Senegal and Vietnam. Journal of Global Antimicrobial Resistance, 19, 222-227. https://doi.org/10.1016/j.jgar.2019.05.002
[6]
Chah, K.F., Ugwu, I.C., Okpala, A., Adamu, K.Y., Alonso, C.A., Ceballos, S., Nwanta, J.N. and Torres, C. (2018) Detection and Molecular Characterisation of Extended-Spectrum β-Lactamase-Producing Enteric Bacteria from Pigs and Chickens in Nsukka, Nigeria. Journal of Global Antimicrobial Resistance, 15, 36-40. https://doi.org/10.1016/j.jgar.2018.06.002
[7]
Yawat Djogang, A.M., Fotsing Kwetché, P.R., Simo Louokdom, J., Gamwo Dongmo, S., Nankam Nguekap, W.L., Tchoukoua, S.H., Kouamouo, J., Njajou, O., Kuiaté, J.R. and Tekam, J.M. (2018) Antibiotic Susceptibility Profile of Bacteria from Farm Wastes: Findings in Chicken Excreta, Food and Water from Four Poultries versus Trend in a Non-Exposed Community of West Cameroon. International Journal of Current Research, 10, 75629-75638.
[8]
Louokdom, S.J., Fotsing Kwetche, P.R., Kouamouo, J., Kengne Toam, A.L., Gamwo, Dongmo, G.S., Tchoukoua, S.H., Tamatcho Kweyang, B.P. and Kuiaté, J.R. (2016) High Antibiotic Resistance in Bacteria from a Healthcare Setting: Case in the Surgery Wards of the Regional Hospital of Bafoussam, West-Cameroon. Journal of Chemical, Biological and Physical Sciences, 6, 1297-307.
[9]
Kwetche, P.R., Louokdom, J.S., Kamga, C., Kaba, K. and Kouamouo, J. (2015) β-Lactamase-Associated Resistance Phenotypes amongst Multidrug Resistant Bacteria Isolated in a School Hospital of West Cameroon. Integrated Journal of British, 2, 59-70.
[10]
Gondam Kamini, M., Tatfo Keutchatang, F., Yangoua Mafo, H., Kansci, G. and Medoua Nama, G. (2016) Antimicrobial Usage in the Chicken Farming in Yaoundé, Cameroon: A Cross-Sectional Study. International Journal of Food Contamination, 3, Article No. 10. https://doi.org/10.1186/s40550-016-0034-6
[11]
Guetiya Wadoum, R.E., Zambou, N.F., Anyangwe, F.F., Njimou, J.R., Coman, M.M., Verdenelli, M.C., Cecchini, C., Silvi, S., Orpianesi, C., Cresci, A. and Colizzi, V. (2016) Abusive Use of Antibiotics in Poultry Farming in Cameroon and the Public Health Implications. British Poultry Science, 57, 483-493. https://doi.org/10.1080/00071668.2016.1180668
[12]
Guyomard-Rabenirina, S., Reynaud, Y., Pot, M., Albina, E., Couvin, D., Ducat, C., Gruel, G., Ferdinand, S., Legreneur, P., Le Hello, S. and Malpote, E. (2020) Antimicrobial Resistance in Wildlife in Guadeloupe (French West Indies): Distribution of a Single blaCTX–M–1/IncI1/ST3 Plasmid among Humans and Wild Animals. Frontiers in Microbiology, 11, Article 1524. https://doi.org/10.3389/fmicb.2020.01524
[13]
Rahmatallah, N., El Rhaffouli, H., Amine, I.L., Sekhsokh, Y., Fihri, O.F. and El Houadfi, M. (2018) Consumption of Antibacterial Molecules in Broiler Production in Morocco. Veterinary Medicine and Science, 4, 80-90. https://doi.org/10.1002/vms3.89
[14]
Bodering, A., Ndoutamia, G., Ngandolo, B.N., Mopate, L.Y. and Ngakou, A. (2017) Characteristics of Poultry Farms and Assessment of Their Level of Contamination by Salmonella spp. and Escherichia coli in the Towns of N’Djamena and Doba in Chad. International Journal of Biological and Chemical Sciences, 11, 1669-1684. https://doi.org/10.4314/ijbcs.v11i4.21
[15]
Maciuca, I.E., Williams, N.J., Tuchilus, C., Dorneanu, O., Guguianu, E., Carp-Carare, C., Rimbu, C. and Timofte, D. (2015) High Prevalence of Escherichia coli-Producing CTX-M-15 Extended-Spectrum Beta-Lactamases in Poultry and Human Clinical Isolates in Romania. Microbial Drug Resistance, 21, 651-662. https://doi.org/10.1089/mdr.2014.0248
[16]
Moffo, F., Mouiche, M.M., Djomgang, H.K., Tombe, P., Wade, A., Kochivi, F.L., Dongmo, J.B., Mbah, C.K., Mapiefou, N.P., Mingoas, J.P. and Awah-Ndukum, J. (2022) Associations between Antimicrobial Use and Antimicrobial Resistance of Escherichia coli Isolated from Poultry Litter under Field Conditions in Cameroon. Preventive Veterinary Medicine, 204, Article ID: 105668. https://doi.org/10.1016/j.prevetmed.2022.105668
[17]
Moffo, F., Mouiche, M.M., Djomgang, H.K., Tombe, P., Wade, A., Kochivi, F.L., Dongmo, J.B., Mbah, C.K., Mapiefou, N.P., Ngogang, M.P. and Awah-Ndukum, J. (2021) Poultry Litter Contamination by Escherichia coli Resistant to Critically Important Antimicrobials for Human and Animal Use and Risk for Public Health in Cameroon. Antibiotics, 10, Article No. 402. https://doi.org/10.3390/antibiotics10040402
[18]
Djuikoue, I.C., Woerther, P.-L., Toukam, M., Burdet, C., Ruppe, E., Gonsu, K.H., et al. (2016) Intestinal Carriage of Extended Spectrum Beta-Lactamase Producing E. coli in Women with Urinary Tract Infections, Cameroon. The Journal of Infection in Developing Countries, 10, 1135-1139. https://doi.org/10.3855/jidc.7616
