Transitioning to Automated Microbiologic Era: Blood Culture Isolates in Children and Adults in Federal Teaching Hospital in Gombe, North East Nigeria 2016-2020
Introduction: Automated blood culture
systems for incubation and growth monitoring have become the standard in
high-income countries (HICs), but are still relatively expensive and not
universally available for implementation in
most low- and middle-income countries (LMIC). We aimed to report blood culture isolates using Automated technique in children and adults admitted into
the Federal Teaching Hospital Gombe from 2016 to 2020. MaterialsandMethods: Blood Culture Isolates in children (0 - 18 years) and adults
(>19 yrs) by Bactec 9050 Automated culture system from 2016-2020 were
retrieved from the medical and laboratory register. Information analyzed
included, age, sex, month, and year and culture growth and reported antibiotic
sensitivity. A Bactec Blood culture tests is $20 in this facility. In Nigeria, the minimum monthly wage is $70 (Official currency exchange rate is
N423/US Dollar). Results: Of the 1713 blood cultures performed, children
0-18years were 1322 (77.2%) and adult (19 years above) (22.8%). Overall
positivity was 733 (42.2%) with males 385 (52.5%). Of the 1322 Blood cultures
(BC) in children 615 (46.5%) were positive for isolates and adults 118 (30.2)%.
Blood culture positivity decreased with
increasing age with newborns 251 (34.5%) and adults > 65 years 18
(2.5%). Staphylococcusaureus constituted 61.3% of all isolates and was the leading isolates in all age
groups; Alkaligenes (9.1%);Citrobacter 8.1%, Klebsiella 6.7%; Pseudomonas 6.1%; E.coli 2.7%;Enterococcus 2%; Proteus 1%. Of the Antimicrobial resistance priority isolates E.coli susceptibility ranged
from 71% to Gentamycin and 100% to Cefixime; Klebsiella from 25% sensitivity to Amikacin to
78% each to chloramphenicol and
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