Introduction:This study aimed to perform routine seminal fluid analysis, sperm DNA fragmentation, and sperm function tests at the chromatin maturation level and evaluate pregnancy in the patients passing intrauterine insemination before starting Intrauterine Insemination (IUI) method. MaterialsandMethods: In this prospective study, 111 couples who underwent Intrauterine Insemination (IUI) in unexplained infertility patients were admitted？to Al-Farah IVF and assisted reproductive center in Baghdad, Iraqbetween November 2020 and February 2021 were evaluated. Semen fluid analysis was performed based on (WHO 4th) guiding rules. In addition, Sperm Chromatin？Dispersion (halo test) and sperm maturation were performed with Aniline？Blue Stain (ABS). Results: Sperm Chromatin Dispersion (SCD)groups were？compared in terms of pregnancy outcome;the positive pregnancy rate was found to be above in the normal SCD groups (p
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