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White Blood Cells Detection Using Spectral Tresholding

DOI: 10.4236/jsip.2023.141001, PP. 1-8

Keywords: Segmentation, Fourier Transform, White Blood Cell

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Abstract:

The study of the morphology of White Blood Cells (WBCs) further contributes to the clinical diagnosis of blood diseases. In this research paper, we come up with an image segmentation enhancement by combining Fourier Fast Transform on smear blood capture and classical thresholding. The Fast Fourier Transform (FFT) is a very powerful tool in image processing and it was used to segment and extract the WBCs. Our image processing method uses a Fast Fourier Transform combined with filtering and an Inverse Fast Fourier Transform for the extraction and visualization of the high frequency region of the image. In order to remove residual Red Blood Cells acting as noise in the expected result, a final thresholding step is added at the end of the processing. The results presented in this article report the tests performed using our mathematical implementation. Moreover, we were able to detect and differentiate the sub-families of WBCs.

References

[1]  Safuan, S.N.M., Tomari, R., Zakaria, W.N.W. and Othman, N. (2017) White Blood Cell Counting Analysis of Blood Smear Images Using Various Segmentation Strategies. AIP Conference Proceedings, 1883, Article ID: 020018.
https://doi.org/10.1063/1.5002036
[2]  Ruberto, C.D., Loddo, A. and Putzu, L. (2019) Detection of Red and White Blood Cells from Microscopic Blood Images Using a Region Proposal Approach. Computers in Biology and Medicine, 116, Article ID: 103530.
https://doi.org/10.1016/j.compbiomed.2019.103530
[3]  Mahmood, N.H. and Mansor, M.A. (2012) Red Blood Cells Estimation Using Hough Transform Technique. Signal & Image Processing: An International Journal (SIPIJ), 3, 53-64.
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[4]  Patil, P.R., Sable, G.S. and Anandgaonkar, G. (2019) Counting of WBCs and RBCs from Blood Images Using Gray Thresholding. International Journal of Research in Engineering and Technology, 3, 391-395.

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