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IAA和GA3强化八宝景天修复Cd污染土壤的研究
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Abstract:
本次研究以八宝景天为植物研究对象，采用盆栽试验模拟镉污染土壤，构建植物激素与超富集植物联合修复试验系统，通过施加赤霉素(GA3)和生长素(IAA)，考察不同浓度的植物激素和植物种类对植物吸收重金属的影响，探究植物激素对景天植物修复镉污染土壤效果。结果表明，与对照组相比，施加植物激素GA3和IAA均可以增加八宝景天的生物量、茎长、根长、叶绿素含量和减少丙二醛的含量。土壤中Cd浓度条件相同的情况下，八宝景天的根长增长效果在叶面喷洒50 mg/L的IAA是最好的；植物茎长增长、鲜重增长和植物叶绿素含量在叶面喷洒50 mg/L的GA3是最多的；植物的MDA含量在叶面喷洒100 mg/L的GA3是最少的。施加不同浓度的植物激素GA3和IAA时，八宝景天对Cd富集效果有不同影响，在土壤中Cd浓度条件相同的情况下，八宝景天根部和地上部分Cd的含量都是在叶面喷洒25 mg/L的GA3时最高。
In this study, the plant study was conducted with Hylotelephium erythrostictum as the plant re-search object, and a pot experiment was used to simulate cadmium-contaminated soil, and a com-bined phytohormone and super-enriched plant remediation test system was constructed to inves-tigate the effect of different concentrations of phytohormones and plant species on plant uptake of heavy metals by applying gibberellin (GA3) and growth hormone (IAA) and investigating the effect of phytohormones on the remediation of cadmium-contaminated soil by Sedum plants. The results showed that the application of both phytohormones GA3 and IAA increased the biomass, stem length, root length, chlorophyll content and reduced malondialdehyde content of Cd in Hylotelephium erythrostictum compared with the control group. Under the same conditions of Cd concentration in the soil, the root length growth of Hylotelephium erythrostictum was best with foliar spraying of 50 mg/L IAA; the plant stem length growth, fresh weight growth and plant chlorophyll content were most with foliar spraying of 50 mg/L GA3; the plant MDA content was least with foliar spraying of 100 mg/L GA3. The Cd enrichment effect of different concentrations of GA3 and IAA in Hylotelephium erythrostictum was different. Under the same conditions of Cd concentration in soil, the highest Cd content in both roots and aboveground parts of Hylotelephium erythrostictum was found in foliar spraying of 25 mg/L GA3.
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